- Open Access
Active induction of experimental autoimmune encephalomyelitis by MOG35-55 peptide immunization is associated with differential responses in separate compartments of the choroid plexus
© Murugesan et al.; licensee BioMed Central Ltd. 2012
- Received: 30 May 2012
- Accepted: 30 July 2012
- Published: 7 August 2012
There is increasing awareness that, aside from producing cerebrospinal fluid, the choroid plexus (CP) might be a key regulator of immune activity in the central nervous system (CNS) during neuroinflammation. Specifically, the CP has recently been posited to control entry of sentinel T cells into the uninflamed CNS during the early stages of neuroinflammatory diseases, like multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). As the CP is compartmentalized into a stromal core containing fenestrated capillaries devoid of typical blood–brain barrier properties, surrounded by a tight junction-expressing choroidal epithelium, each of these compartments might mount unique responses that instigate the neuroinflammatory process.
To discern responses of the respective CP stromal capillary and choroidal epithelial tissues during evolving neuroinflammation, we investigated morphology and in situ expression of 93 immune-related genes during early stages of EAE induced by immunization with myelin oligodendrocyte glycoprotein peptide (MOG35-55). Specifically, 3-D immunofluorescent imaging was employed to gauge morphological changes, and laser capture microdissection was coupled to an Immune Panel TaqMan Low Density Array to detail alterations in gene expression patterns at these separate CP sites on days 9 and 15 post-immunization (p.i.). To resolve CP effects due to autoimmunity against MOG peptide, from those due to complete Freund’s adjuvant (CFA) and pertussis toxin (PTX) included in the immunization, analysis was performed on MOG-CFA/PTX-treated, CFA/PTX-treated, and naïve cohorts.
The CP became swollen and displayed significant molecular changes in response to MOG-CFA/PTX immunization. Both stromal capillary and choroidal epithelial tissues mounted vigorous, yet different, changes in expression of numerous genes over the time course analyzed - including those encoding adhesion molecules, cytokines, chemokines, statins, interleukins, T cell activation markers, costimulatory molecules, cyclooxygenase, pro-inflammatory transcription factors and pro-apoptotic markers. Moreover, CFA/PTX-treatment, alone, resulted in extensive, though less robust, alterations in both CP compartments.
MOG-CFA/PTX immunization significantly affects CP morphology and stimulates distinct expression patterns of immune-related genes in CP stromal capillary and epithelial tissues during evolving EAE. CFA/PTX treatment, alone, causes widespread gene alterations that could prime the CP to unlock the CNS to T cell infiltration during neuroinflammatory disease.
- Laser capture microdissection (LCM)
- Choroid plexus
- Pertussis toxin
Though the choroid plexus (CP) is commonly recognized as the production site of cerebrospinal fluid (CSF) [1–3], it has relatively recently gained attention as a critical player in central nervous system (CNS) inflammation [4–6]. Specifically, the CP has been suggested as the site of entry into the uninflamed CNS of pioneer T cells searching for their cognate antigens during immunosurveillance and in the early stages of neuroinflammatory diseases such as multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE) [7, 8]. Current theory holds that, after crossing the CP into the CSF, pioneer T cells travel to the subarachnoid space (SAS), where antigen-presenting cells reactivate them. In turn, reactivation is thought to set off a burst of cytokines and other mediators that inflames meningeal and parenchymal venules to initiate disease [9–11].
The anatomy of the CP appears well suited to orchestrating the initial steps of CNS inflammation. It projects from the roofs of all four ventricles into the CSF, and is composed of two distinct tissue layers: a highly vascularized stroma encapsulated by a “tight” layer of epithelial cells . Unlike the parenchymal capillaries forming the restrictive blood–brain barrier (BBB), CP stromal capillaries are fenestrated and contain pentilaminar junctions whose outer leaflets are not fused  – properties that render the CP capillary population highly permeable to macromolecules . This juxtaposition of “leaky” capillaries and tight epithelium constitutes the blood-cerebrospinal fluid barrier (BSCFB) [15, 16], an arrangement construed as enabling blood-born leukocytes to extravasate into an uninflamed brain during the incipient stages of MS and EAE [17, 18]. Supporting this process, expression of chemokine CCL20 by choroidal epithelial cells is thought to chemotactically draw T cells – bearing the cognate receptor CCR6 – from the CP stroma, across the epithelium and into the CSF .
In situ hybridization and immuno-electron microscopy of the CP has further revealed expression of adhesion molecules, VCAM-1 and ICAM-1, by choroidal epithelial cells of the healthy CP, and additionally of MAdCAM-1 by these same cells during EAE [19, 20]. Also, transcriptome analysis of the whole adult CP has highlighted expression of immune mediators in both healthy mice  and those subject to peripheral inflammation , reinforcing the view this organ is a critical conduit linking immune/inflammatory activities between the periphery and CNS. But the extremely close apposition of the different CP layers has posed a significant challenge to studying the depth of their respective contributions to inflammatory processes. In fact, no immune function has yet been ascribed to the CP capillary endothelium, leaving completely unresolved the factors that drive T cell emigration into the stroma. And gene regulatory events surrounding transmigration of T cells across the choroidal epithelium further remain unsettled.
To elaborate the sequence of events in the CP that set the stage for CNS inflammation during EAE induced by active immunization with MOG35-55 peptide, we used laser capture microdissection (LCM) coupled to qrt-PCR-based microarray  to establish the time course of expression of a panorama of immune mediators in the separate stromal (including capillaries) and choroid epithelial layers. Morphological changes in the CP associated with MOG immunization were also examined by quantitative 3-D image analysis following confocal microscopy. Results reveal substantial changes in CP gene expression and morphology occurred in response to vspecific aspects of the MOG immunization process. These results could hold relevance for how combinations of environmental factors trigger neuroinflammatory disease.
Female C57BL/6 mice, age 8–10 weeks and obtained from Charles River Laboratories, Inc. (Wilmington, MA), were used to minimize microvascular heterogeneity due to genetic variability, sex, and age . Animals were euthanized by CO2 inhalation, following Animal Care and Use Guidelines of the University of Connecticut Health Center (Animal Welfare Assurance # A3471-01). A total of n = 3 animals/group were used for each treatment and time-point assessed.
Induction of experimental autoimmune encephalomyelitis (EAE)
EAE was induced in mice by active immunization with MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK), of murine origin (W. M. Keck Biotechnology Resource Center, Yale University), as described ; following Animal Care and Use Guidelines of the University of Connecticut Health Center (Animal Welfare Assurance # A3471-01). Briefly, on day 0, one group of female mice 7–9 weeks of age was injected subcutaneously with 300 μg of MOG peptide in complete Freund’s adjuvant (CFA, DIFCO) into the right and left flank, 100 μl per site. These mice were also injected i.p. with 500 ng pertussis toxin (PTX, List Laboratories, Campbell CA) in PBS on days 0 and 2 following the first immunization (referred to as the MOG-CFA/PTX group). The second group of age-matched mice received CFA alone and PTX (500 ng) injections on day 0 and a second injection of 500 ng PTX alone on day 2 (referred to as the CFA/PTX group). The third group of naïve age-matched female mice was left untreated. Animals were monitored and scored daily for clinical disease severity according to the following scale: 0 = normal; 1 = tail limpness; 2 = limp tail and weakness of hind legs; 3 = limp tail and complete paralysis hind legs; 4 = limp tail, complete hind leg and partial front leg paralysis; and 5 = death. LCM tissue was acquired at day 9 (score 0) and day 15 (score ~ 2.0) post-immunizations.
Tissue preparation for Immuno-LCM
Brains were snap-frozen in dry ice-cooled 2-methylbutane (Acros; Geel, Belgium), and stored at −80°C. Frozen brain was embedded in cryomatrix compound (Thermo Fisher Scientific, Waltham, MA) prior to sectioning. Coronal sections (7 μm) were cut on a Microm HM 505 M cryostat (Mikron Instruments; Oakland, NJ) and affixed to uncoated, pre-cleaned glass slides (Fisher Scientific, Pittsburgh, PA) and stored in a slide box at −80°C. Tissue was processed for LCM within a week of sectioning.
Immunostaining for Immuno-LCM
Immunostaining was performed as detailed [24, 26, 27], with minor modifications. Briefly, sections were fixed in 75% ethanol, on ice, for 3 min prior to staining. The CP stromal capillaries were stained using alkaline phosphatase substrate NBT (nitro-blue tetrazolium chloride)/BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt), (Vector Labs, Burlingame, CA) for 3–5 minutes in 100 Mm Tris–HCl (pH 9.5) to detect endogenous alkaline phosphatase activity in the endothelial cells. In this case, endothelial cells were intentionally not immunostained by anti-CD31/ABC alkaline phosphatase [24, 27], as it resulted in extensive deposition of chromogenic precipitate, which made the stromal capillaries difficult to resolve from the choroidal epithelial layer. The choroidal epithelial cells were immunostained with monoclonal pan-cytokeratin-FITC antibody (Sigma) for 10 minutes (diluted 1:10 in 1X PBS + 0.5% Tween-20). RNAsin® RNAse inhibitor (Promega, Madison, WI) was added to all staining reagents. Immediately after immunostaining, sections were dehydrated through graded alcohol and xylenes as described .
Laser capture microdissection (LCM)
A PixCell IIe laser capture microscope (ABI, Foster City, CA) was used to separately procure CP stromal capillary and CP choroidal epithelial tissues, as previously described for brain parenchymal vessels [24, 27, 28]. We refer specifically to CP stromal capillary tissue, instead of pure capillary endothelium, as it was not possible to completely resolve vascular from matrix elements (including extravasating leukocytes) within the dense CP stroma. Likewise, the choroidal epithelial tissue may contain some epiplexus cells, and so is not described as pure epithelium. Only choroid plexus material from within the fourth ventricle and lateral recess of the fourth ventricle was retrieved.
LCM-retrieved tissue was solubilized in Cell Lysate Buffer® (Signosis; Sunnyvale, CA) for direct reverse transcription. Cell Lysate Buffer®, pre-heated to 75°C, was added and the resulting lysate heated at 75°C for an additional 15 min. Samples were immediately frozen at −80°C.
DNase treatment and cDNA synthesis
Cell Lysate Buffer® extracts were treated with Turbo DNase (Ambion; Austin, TX) according to the manufacturer’s instructions. Specifically, Turbo DNase buffer and DNase were added and samples incubated at 37°C for 30 min. Next, DNAse inactivation reagent was added for 2 min at room temperature. Samples were then reverse transcribed using the SuperScript III (Invitrogen) standard protocol with random hexamers (Roche; Indianapolis, IN), and employing an extension temperature of 42°C – optimal for random hexamers – for 60 min. Resulting cDNA was stored at −20°C until used for analysis.
Pre-amplification was carried for array analysis out using TaqMan® PreAmp Master Mix and a PreAmp Pool containing all the primers for detection by the Mouse Immune Panel TaqMan® Low density Array (TLDA; Life Technologies Corp., Foster City, CA) . This panel conatins 93 immune-related genes plus three housekeeping control genes (see Additional file 1: Mouse Immune Panel TLDA). Pre-amplification was carried out with an initial hold at 95°C for 10 min, followed by 14 cycles at 95°C for 15 sec and 60°C for 4 min.
Relative cDNA levels were quantified by qrt-PCR using an ABI PRISM 7500 Sequence Detection System Version 2.3, and reported compared to housekeeping gene GAPDH. Relative quantitation to GAPDH was performed using the standard 2–δCt method of Pfaffl , where δCt = Ct target threshold cycle – Ct reference (GAPDH) threshold cycle. Expression of genes relative to GAPDH was then represented as percent expression of GAPDH. To assure consistency in relating gene expression patterns to a housekeeping gene, GAPDH and two other housekeeping genes, β-actin and 18 S ribosomal RNA, were evaluated for constant expression across treatments. Additionally, gene expression values for a handful of randomly selected immune-related genes were also determined relative to β-actin and 18 S ribosomal RNA (see Additional file 2: Housekeeping control genes). Custom TaqMan® primers/probes were used for the Mouse Immune Panel TLDA. TLDA analysis was conducted as per the manufacturer’s protocol, with 100 μl sample volumes containing a 1/32 dilution of pre-amplified cDNA added to each port of the microfluidic card . For qrt-PCR analysis of CD31 and Cytokeratin 8, ‘singleplex’ assays were used as neither of these genes are represented in the mouse Immune Panel TLDA.
Immunostaining for confocal microscopy
Frozen cryosections (60 μm) were fixed with 4% paraformaldehyde, permeabilized with 1% Triton X-100 (in PBS) and incubated with Powerblock® for 10 min. Purified rat anti-mouse CD31 antibody (BD Pharmingen; 1:150 dilution in 10% NBS in 1X PBS + 0.5% TW-20) was used to stain the CP capillary network followed by incubation with goat anti-rat Alexa-555 secondary antibody (1:200). Pan-cytokeratin-FITC 1: 150 dilution, (Sigma) was used to stain the CP epithelium. Next, Alexa-647 anti-mouse CD45 antibody (1:160 dilution) was used to stain leukocytes.
Images were acquired on a Zeiss LSM 510 Meta laser scanning confocal microscope, and optical slices (at 2-μm intervals) obtained using a 40x objective. Acquired z-stacks were background-subtracted, and 3-D isosurface rendering performed using Bitplane IMARIS suite version 7.1 x 64 software (Bitplane Inc. Saint Paul, MN). Each z-stack was thresholded and the “filament tracker” module used to generate a 3-D traced outline of immunostained vessels in order to determine the diameter range of the CD31-immunostained capillary network within the choroid plexus across different treatments.
Relative gene expression values are given as mean ± SEM. Student’s two-tailed test (Microsoft Excel 2003, Redmond, WA) was employed to assess statistical significance in gene expression values between MOG-CFA/PTX and CFA/PTX samples from the CP capillary stroma and CP epithelium groups, separately for the two different time points assessed. Results were considered significant at a p ≤ 0.05. Additionally, two-way ANOVA followed by post-hoc Bonferroni analysis was performed using GraphPad Prism 5 (GraphPad, La Jolla, CA) to determine interactive effects between immunization treatment and time of analysis post-immunization, and assessed for each CP compartment.
Anatomy of the CP is altered in response to MOG-immunization
LCM enables resolution of CP stromal capillaries from the choroidal epithelium
Expression of immune-related genes by stromal CP capillary tissue following immunization
The next series of experiments coupled LCM to TLDA qrt-PCR arrays to further characterize expression patterns of a panorama of 93 immune-related genes in the separate CP compartments at different stages of the neuroinflammatory response to immunization. Expression of these genes relative to housekeeping gene GAPDH (GAPDH was unaffected across treatments; Additional file 2: Housekeeping control genes), was determined in three groups of mice: MOG-CFA/PTX, CFA/PTX and naïve at two time points: day 9 and 15 p.i. Contrasting these three treatment groups enabled effects of the adjuvants CFA and PTX to be distinguished from the autoimmune response to MOG. Furthermore, examining effects at day 9 p.i. (prior to any evidence of clinical disease) and day 15 p.i. (after disease onset), highlighted the progression of gene changes that may be linked with developing pathology.
Genes similarly up-regulated in CP stromal capillary tissue from both MOG-CFA/PTX- and CFA-PTX-immunized mice at day 9 p.i
Genes modulated similarly in stromal CP capillary of MOG-CFA/PTX mice at day 9 p.i
Notably, the CFA/PTX group indicated some dampening of immune regulation by this time, as certain genes e.g., Smad3 and Gzmb, dropped back to levels matching those of naïve mice, after initially displaying an elevation at day 9. Genes that trended towards elevated expression following MOG-CFA/PTX treatment (but with p values slightly > 0.05) are displayed in Additional file 3: Genes that trended towards elevated expression in MOG-CFA/PTX- immunized CP stromal capillary tissue compared to CFA-PTX-immunized mice, at day 15 p.i, while those that were similarly up-regulated in CP stromal capillary tissue of MOG-CFA/PTX- and CFA/PTX-immunized mice compared to naïve mice at day 15 are listed in Additional file 4: Genes similarly up-regulated in CP stromal capillary tissue from both MOG-CFA/PTX- and CFA-PTX-immunized mice at day 15 p.i. Genes that were undetected in the CP capillary tissue in all treated and naïve mice at both time-points were the following: CCR4, CD19, CD3e, CSF3, Ctla4, Cyp1a2, Cyp7a1, H2-Ea, IL12b, IL13, IL3, IL4, IL5, IL6, IL9, and Lta.
Expression of immune-related genes by CP choroidal epithelium following immunization
Immunization also produced a change in expression of numerous immune-related genes within the CP choroidal epithelium. Moreover, these changes differed from those observed in the capillary stroma, emphasizing the differential immune sensitivities of the two tissues.
By day 15 p.i., genes B2m and CXCL10 displayed further increases in expression in the CP choroidal epithelium of MOG-CFA/PTX mice compared to that seen in this cohort at day 9 (Figure 4B), showing > 19-fold and > 800-fold higher levels, respectively, compared to naïve mice. Expression levels of yet additional genes in MOG-treated mice also became elevated by this time; these included Bax, Bcl2l1, C3, CD68, Gusb, H2-Eb1 and Ski (Figure 4B). Moreover, genes CCL19, CCL2, CCR2, CD8a, Sele and Selp, which had previously shown up-regulation only in the MOG-CFA/PTX group at day 9, became similarly induced in the CFA/PTX group at this later time-point. Genes that trended towards elevated expression following MOG-CFA/PTX treatment for both time points (but with p values slightly >0.05) are displayed in Additional file 5 and Additional file 6: Genes that trended towards elevated expression in MOG-CFA/PTX immunized CP epithelium tissue compared to CFA-PTX-immunized mice, at day 9/15p.i., while those that were similarly up-regulated in CP choroidal epithelial tissue of MOG-CFA/PTX- and CFA/PTX-immunized mice compared to naïve mice for both time points are listed in Additional file 7 and Additional file 8: Genes similarly up-regulated in CP epithelium from both MOG-CFA/PTX- and CFA-PTX-immunized mice at day 9/15 p.i. Those few genes that were in the CP epithelial tissue in both immunized groups and naïve mice included IL3, IL4, IL5, IL6, Lta.
Interaction between immunization treatment and time
Due to increasing awareness of the CP as fundamental to the development of CNS inflammation [4–6], immuno-LCM coupled to qrt-PCR array was used to separately acquire CP stromal capillary and choroidal epithelial tissues and assess their respective patterns of expression in situ of a wide panorama of immune-related genes. Gene patterns were evaluated during preclinical and early clinical stages of EAE to appreciate the switches in gene expression that accompany evolving disease.
It is clear that the CP responds vigorously to MOG immunization at both the anatomical and molecular levels. Interestingly, immunization with CFA and PTX alone produced striking effects. Swelling of the capillary plexus occurred to nearly the same extent with injection of just these agents, as with PTX and MOG in CFA. PTX is an ancillary adjuvant commonly employed to elicit EAE, as well as several other experimental autoimmune diseases [40–44]. And while its mechanism of action in this regard has generally been attributed to increasing vascular permeability [45–47] – most notably that of the BBB [48–51] – additional hypotheses have been put forth [52–55]. However, to the best of our knowledge, this is the first report to turn attention to the CP as a possible target of PTX. It is of further interest to point out that the distension of CP capillaries noted here study bears similarity to that seen following systemic neutralization of VEGF and TGFβ . In the latter case, CP capillary swelling was accompanied by loss of fenestrae from endothelial cells and appearance of multiple caveolae, transport vesicles that transcytose a variety of cargo  – including chemokines  – and are often associated with heightened vascular permeability and inflammation [59, 60]. Engelhardt et al.  had also described ultrastructural changes of the CP during EAE (along with CFA and PTX as adjuvants), but noted these were mostly restricted to the CP choroidal epithelium. Moreover, as comparison in this latter study was just between healthy mice and those afflicted with EAE, it is unclear whether the observed epithelial response was autoimmune in nature and/or due to adjuvant action.
Our results suggest that injection of PTX and/or CFA, alone, might trigger an immune response in the CP capillaries that helps “set the stage” for CNS inflammation . The CP capillaries might be uniquely responsive in this regard, as CFA/PTX treatment evoked an early response (day 9 p.i.) in the CP stromal capillary tissue, while the choroidal epithelium experienced neither overt morphological nor gene expression changes at this time. If, as speculated during MS/EAE, Th17 cells first transit through the CP, and then travel in the CSF to reach their cognate antigens in the SAS, then the CP capillaries must somehow initially be rendered capable of supporting T cell extravasation. In the EAE paradigm used here, PTX and/or CFA might provide the stimulus to evoke such capability. In this regard, we noted > 10-fold increase in chemokines CCL2, CCL5 and CXCL10 in the CP stromal capillaries of both MOG-CFA/PTX- and CFA/PTX-treated mice at day 9 p.i. Constitutive CCL2 expression within the CP stromal tissue has been reported using in situ hybridization analysis, and shown to be induced following peripheral tissue inflammation . The ability of CFA/PTX treatment to stimulate expression of these chemokines could potentially reflect the actions of one or both of these adjuvants to ‘prime’ the neuroinflammatory process by activating the endothelium to elicit initial auto reactive T cell extravasation from the circulation into the stromal compartment. This hypothesis is consistent with the recent observation that administration of PTX to transgenic mice over-expressing CCL2 in the CNS causes disruption of the BBB and promotes leukocyte migration into the brain parenchyma .
Notwithstanding the effects of CFA/PTX treatment on CP capillary morphology and gene expression, immunization with MOG-CFA/PTX further induced the expression of additional genes – some or all of which might specifically reflect the autoimmune response and associated development of EAE. While perhaps necessary for disease to develop, the CP conditions set in place by PTX and CFA are insufficient for inducing EAE in wild-type C57BL/6 mice. For disease to occur, supernumerary induction of some genes, and de novo induction of others must take place. The findings by Goverman et al. and Brabb et al. [61, 64], that injection of PTX alone can “trigger” EAE in TCR-transgenic mice specific for myelin basic protein, by fostering T cell access to the CNS, comports with our results and the view that PTX enables mice to reach the disease threshold. And helping pull this trigger may be additional effects of PTX on T cell behavior. Our observation of increased mRNA for genes CD8a, CD80, CD86, Gzmb (granzyme) and Ptprc (CD45) in the CP capillary stromal tissue of both MOG-CFA/PTX and CFA/PTX cohorts may reflect capture of PTX-activated CD8 T cells in transit across the CP and into the CSF. This interpretation is consistent with the recent finding by Murphey et al. , that PTX stimulation of cultured spleen cells results in CD 8 T cell activation via CD80/86 co-stimulation.
As to signals responsible for the extravasation of T cells from the circulation into the CP stroma during MOG-induced EAE, a combination of chemokines may fill this role, as these immune mediators do in guiding parenchymal leukocyte infiltration. In particular, CCL5 level was increased significantly in CP stromal capillary tissue at day 15 p.i., which coincides with high CCL5 protein level reported in whole brain extract of EAE mice at a similar time-point, and argued to mediate leukocyte adherence to the CNS microvasculature . And CCL19 – a CCR7 ligand known to play a crucial role in EAE development through IL-23 producing Th17 cells  – was likewise up-regulated dramatically in the CP stromal capillaries of MOG immunized mice at day 15 p.i.
With specific regard to those mechanism(s) further driving T cell migration from the CP stroma into the CSF, recent evidence points toward expression of another chemokine - CCL20 - by the CP choroidal epithelium as directing CCR6+ T cells across this layer and into ventricular fluid during MS/EAE . However, as this chemokine:cognate receptor pair was not represented on the commercial TLDA card used in these experiments, confirmation of this pathway was not performed. Aside from CCL20 providing a driving force for T cell migration into CP epithelium, CCL2 might also serve in this capacity, as Chodobska et al.  noted the latter chemokine was rapidly stimulated in this tissue in vivo and then released into the CSF, following traumatic brain injury. Indeed, the significant increase in CCL2 we observed in the MOG-CFA/PTX CP choroidal epithelium at day 9 p.i. might just reflect such a role for this chemokine in EAE. The cognate receptor for CCL2, CCR2 was also seen to be elevated in the choroidal epithelium at this early time-point. Presently, it is unclear if the high levels of CCR2 mRNA indicate activation of the epithelium, which, in the periphery, has been shown to express CCR2 [68, 69], or the accumulation of infiltrating CCR2+ T cells.
In what might suggest multi-level control of leukocyte extravasation into the CSF, still other chemokines were also significantly up-regulated by the CP during EAE – namely CXCL10 and CCL19. As CXCL10 has been reported to be up-regulated in the sub-ventricular zone (SVZ) during EAE, and postulated to stimulate migration of activated T cells into the SVZ , its spike in expression by CP choroidal epithelial tissue at day 9 p.i. and more robust elevation by day 15 p.i., might imply this chemokine is obligate for T cell entry into the ventricles. In analogous manner, CCL19 was also elevated at this site at day 9 p.i. In fact, the timing of the CP epithelial spikes in this chemokine during early stages of EAE noted here, coincides well with that reported by Reboldi et al.  for initial T cell entry into the uninflamed CNS through the CP. Recently, Marques et al. [22, 71] used hybridization-based microarray to assess the global transcriptome of the whole CP following chronic peripheral LPS stimulation. When compared to our study, there were some common and unique findings. Among the common findings, complement protein C3, and chemokines CCL2 and CCL5 were elevated following either acute or chronic peripheral LPS stimulation, as well as during MOG-induced EAE (complement C3 in both CP capillary tissue and epithelium; CCL2 in CP epithelium; and CCL5 in CP capillary tissue). And Selectin (Sele and Selp) expression was also elevated both following acute peripheral LPS stimulation , and in the CP epithelium after MOG-induced EAE. These common gene modulations may thus reflect more generic CP inflammatory response genes. As for unique findings, these too involved chemokines. Marques et al.  reported stimulation of CCL7 and CXCL1 in the CP following chronic LPS stimulation, while we detected stimulation of CCL19 and CXCL10 in the CP epithelium and CCL19 in the CP endothelium during MOG-induced EAE. A priori, up-regulation of these latter two chemokine genes may more distinguish an EAE signature for the respective CP tissue compartments.
Of further note was our observation of a MOG-sensitive increase in expression of B2m at day 15 p.i. in CP stromal capillary tissue, and at both time-points in the choroidal epithelial tissue. Aside from perhaps reinforcing a more ubiquitous role for B2m in inflammation , this result complements previous reports of increased B2m mRNA and protein levels in both neuronal and non neuronal cell types during EAE [72, 73], a response thought to be due to induction of synaptic plasticity by infiltrating autoreactive immune cells. The sharp induction of complement C3, also noted in both CP compartments of MOG-CFA/PTX mice at the later time point (day 15 p.i), further supports previous studies highlighting C3 deficiency inhibits development of EAE .
As to the specific approach used here, i.e., LCM coupled to TLDA, it offered extraordinary opportunity to probe, in extensive detail, the focused immune response within the distinct CP compartments. Earlier reports, using immunohistochemistry and in situ hybridization, had shown that the respective CP stromal capillary endothelial cells and the CP choroidal epithelial cells displayed different expression patterns of a small nucleus of adhesion molecules during EAE [4, 20]. Specifically, VCAM-1 and ICAM-1 were found to be expressed constitutively by CP choroidal epithelial cells of healthy SJL/N mice, and then further induced following active immunization with spinal cord homogenate. MAdCAM-1 was only seen in these cells after induction of EAE. However, none of these adhesion molecules, nor E- or P-selectin, was detected in CP stromal capillary endothelial cells .
We too noted constitutive VCAM-1 expression in the CP choroidal epithelial tissue of healthy naïve mice. Likewise, VCAM-1 trended toward elevation within this CP compartment of MOG-CFA/PTX cohorts at both days 9 and 15 p.i., though it showed no up-regulation in CFA-PTX-immunized mice at either time-point. In further agreement with previous observations , our analysis demonstrated induction of E-selectin and P-selectin in the CP choroidal epithelial tissue from MOG-CFA/PTX-immunized mice at day 9. Our results nevertheless displayed some stark differences with earlier reports. Specifically, we also noted a trend of increased VCAM-1 expression by day 15 in CP stromal capillary tissue with MOG immunization, paralleling what has been described in MS brain tissue . And both E- and P-selectin mRNA were also observed to be induced in CP stromal capillary tissue of both MOG-CFA/PTX- and CFA/PTX-immunized mice compared to that of naïve mice at day 9 p.i. E-selectin increased expression in the two immunized groups by >100-fold, while P-selectin was stimulated >10-fold.
A priori, differences in results between these EAE studies could result from several factors, among them being 1) the EAE model employed (e.g., immunization of SJL/6 mice with spinal cord homogenate versus immunization of C57BL/6 mice with MOG35-55 peptide), the time of analysis post-immunization (e.g, before or after disease onset), and 3) the sensitivity of the analytic techniques (e.g., in situ hybridization versus qrt-PCR). As neither MAdCAM-1 nor ICAM-1 were represented on the TLDA card used in these experiments, confirmation of expression or lack thereof was not possible for these genes.
Most recently, Liddelow et al.  employed LCM to collect mouse lateral ventricular CP tissue CP for transcriptome analysis of transporter gene expression during normal development. Here, we extended this application, utilizing LCM to resolve – for the first time – the CP capillary stromal tissue from the CP choroidal tissue, and then separately analyzing each for their unique immune responses to MOG immunization.
Induction of EAE in C57BL/6 mice by active immunization with MOG35-55 peptide results in the respective CP stromal capillary and choroidal epithelial compartments each mounting vigorous, yet distinct, immune responses, underscoring the active role of the CP in instigating CNS inflammatory disease. Furthermore, our results make clear that a significant component of the total CP response is due to effects elicited by adjuvants PTX and/or CFA used in the immunization protocol – which might serve to prime the CP to support autoimmune activity necessary for developing MS/EAE. These results are summarized schematically in Figure 6.
This work was supported by grant RG 4503A4/1 from the National Multiple Sclerosis Society to J. S. Pachter and S. Ge.
- Davson H, Segal MB: The effects of some inhibitors and accelerators of sodium transport on the turnover of 22Na in the cerebrospinal fluid and the brain. J Physiol. 1970, 209: 131-153.PubMedPubMed CentralView ArticleGoogle Scholar
- Speake T, Whitwell C, Kajita H, Majid A, Brown PD: Mechanisms of CSF secretion by the choroid plexus. Microsc Res Tech. 2001, 52: 49-59. 10.1002/1097-0029(20010101)52:1<49::AID-JEMT7>3.0.CO;2-C.PubMedView ArticleGoogle Scholar
- Brown PD, Davies SL, Speake T, Millar ID: Molecular mechanisms of cerebrospinal fluid production. Neuroscience. 2004, 129: 957-970.PubMedPubMed CentralView ArticleGoogle Scholar
- Engelhardt B, Wolburg-Buchholz K, Wolburg H: Involvement of the choroid plexus in central nervous system inflammation. Microsc Res Tech. 2001, 52: 112-129. 10.1002/1097-0029(20010101)52:1<112::AID-JEMT13>3.0.CO;2-5.PubMedView ArticleGoogle Scholar
- Brown DA, Sawchenko PE: Time course and distribution of inflammatory and neurodegenerative events suggest structural bases for the pathogenesis of experimental autoimmune encephalomyelitis. J Comp Neurol. 2007, 502: 236-260. 10.1002/cne.21307.PubMedView ArticleGoogle Scholar
- Vercellino M, Votta B, Condello C, Piacentino C, Romagnolo A, Merola A, Capello E, Mancardi GL, Mutani R, Giordana MT, Cavalla P: Involvement of the choroid plexus in multiple sclerosis autoimmune inflammation: a neuropathological study. J Neuroimmunol. 2008, 199: 133-141. 10.1016/j.jneuroim.2008.04.035.PubMedView ArticleGoogle Scholar
- Kivisakk P, Mahad DJ, Callahan MK, Trebst C, Tucky B, Wei T, Wu L, Baekkevold ES, Lassmann H, Staugaitis SM, Campbell JJ, Ransohoff RM: Human cerebrospinal fluid central memory CD4+ T cells: evidence for trafficking through choroid plexus and meninges via P-selectin. Proc Natl Acad Sci USA. 2003, 100: 8389-8394. 10.1073/pnas.1433000100.PubMedPubMed CentralView ArticleGoogle Scholar
- Reboldi A, Coisne C, Baumjohann D, Benvenuto F, Bottinelli D, Lira S, Uccelli A, Lanzavecchia A, Engelhardt B, Sallusto F: C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat Immunol. 2009, 10: 514-523.PubMedView ArticleGoogle Scholar
- Kivisakk P, Imitola J, Rasmussen S, Elyaman W, Zhu B, Ransohoff RM, Khoury SJ: Localizing central nervous system immune surveillance: meningeal antigen-presenting cells activate T cells during experimental autoimmune encephalomyelitis. Ann Neurol. 2009, 65: 457-469. 10.1002/ana.21379.PubMedPubMed CentralView ArticleGoogle Scholar
- Bartholomaus I, Kawakami N, Odoardi F, Schlager C, Miljkovic D, Ellwart JW, Klinkert WE, Flugel-Koch C, Issekutz TB, Wekerle H, Flugel A: Effector T cell interactions with meningeal vascular structures in nascent autoimmune CNS lesions. Nature. 2009, 462: 94-98. 10.1038/nature08478.PubMedView ArticleGoogle Scholar
- Goverman J: Autoimmune T cell responses in the central nervous system. Nat Rev Immunol. 2009, 9: 393-407. 10.1038/nri2550.PubMedPubMed CentralView ArticleGoogle Scholar
- Wolburg H, Paulus W: Choroid plexus: biology and pathology. Acta Neuropathol. 2010, 119: 75-88. 10.1007/s00401-009-0627-8.PubMedView ArticleGoogle Scholar
- Brightman M: Ultrastructural characteristics of adult choroid plexus: Relation to the blood-cerebral spinal fluid barrier to proteins. The Choroid Plexus in Health and Disease. Edited by: Netsky MG. 1975, University Press of Virginia Charlottesville, VA, 86-112.View ArticleGoogle Scholar
- Hurley JV, Anderson RM, Sexton PT: The fate of plasma protein which escapes from blood vessels of the choroid plexus of the rat–an electron microscope study. J Pathol. 1981, 134: 57-70. 10.1002/path.1711340107.PubMedView ArticleGoogle Scholar
- Redzic ZB, Segal MB: The structure of the choroid plexus and the physiology of the choroid plexus epithelium. Adv Drug Deliv Rev. 2004, 56: 1695-1716. 10.1016/j.addr.2004.07.005.PubMedView ArticleGoogle Scholar
- Johanson CE, Stopa EG, McMillan PN: The blood-cerebrospinal fluid barrier: structure and functional significance. Methods Mol Biol. 2011, 686: 101-131. 10.1007/978-1-60761-938-3_4.PubMedView ArticleGoogle Scholar
- Axtell RC, Steinman L: Gaining entry to an uninflamed brain. Nat Immunol. 2009, 10: 453-455. 10.1038/ni0509-453.PubMedView ArticleGoogle Scholar
- Ransohoff RM: Immunology: In the beginning. Nature. 2009, 462: 41-42. 10.1038/462041a.PubMedView ArticleGoogle Scholar
- Steffen BJ, Breier G, Butcher EC, Schulz M, Engelhardt B: ICAM-1, VCAM-1, and MAdCAM-1 are expressed on choroid plexus epithelium but not endothelium and mediate binding of lymphocytes in vitro. Am J Pathol. 1996, 148: 1819-1838.PubMedPubMed CentralGoogle Scholar
- Wolburg K, Gerhardt H, Schulz M, Wolburg H, Engelhardt B: Ultrastructural localization of adhesion molecules in the healthy and inflamed choroid plexus of the mouse. Cell Tissue Res. 1999, 296: 259-269. 10.1007/s004410051287.PubMedView ArticleGoogle Scholar
- Marques F, Sousa JC, Coppola G, Gao F, Puga R, Brentani H, Geschwind DH, Sousa N, Correia-Neves M, Palha JA: Transcriptome signature of the adult mouse choroid plexus. Fluids Barriers CNS. 2011, 8: 10-10.1186/2045-8118-8-10.PubMedPubMed CentralView ArticleGoogle Scholar
- Marques F, Sousa JC, Coppola G, Falcao AM, Rodrigues AJ, Geschwind DH, Sousa N, Correia-Neves M, Palha JA: Kinetic profile of the transcriptome changes induced in the choroid plexus by peripheral inflammation. J Cereb Blood Flow Metab. 2009, 29: 921-932. 10.1038/jcbfm.2009.15.PubMedView ArticleGoogle Scholar
- Demarest TG, Murugesan N, Shrestha B, Pachter JS: Rapid expression profiling of brain microvascular endothelial cells by immuno-laser capture microdissection coupled to TaqMan((R)) Low Density Array. J Neurosci Methods. 2012, 206: 200-204. 10.1016/j.jneumeth.2012.02.023.PubMedPubMed CentralView ArticleGoogle Scholar
- Macdonald JA, Murugesan N, Pachter JS: Validation of immuno-laser capture microdissection coupled with quantitative RT-PCR to probe blood–brain barrier gene expression in situ. J Neurosci Methods. 2008, 174: 219-226. 10.1016/j.jneumeth.2008.07.009.PubMedView ArticleGoogle Scholar
- Juedes AE, Hjelmstrom P, Bergman CM, Neild AL, Ruddle NH: Kinetics and cellular origin of cytokines in the central nervous system: insight into mechanisms of myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis. J Immunol. 2000, 164: 419-426.PubMedView ArticleGoogle Scholar
- Kinnecom K, Pachter JS: Selective capture of endothelial and perivascular cells from brain microvessels using laser capture microdissection. Brain Res Brain Res Protoc. 2005, 16: 1-9. 10.1016/j.brainresprot.2005.08.002.PubMedView ArticleGoogle Scholar
- Macdonald JA, Murugesan N, Pachter JS: Endothelial cell heterogeneity of blood–brain barrier gene expression along the cerebral microvasculature. J Neurosci Res. 2010, 88: 1457-1474.PubMedGoogle Scholar
- Murugesan N, Macdonald JA, Lu Q, Wu SL, Hancock WS, Pachter JS: Analysis of mouse brain microvascular endothelium using laser capture microdissection coupled with proteomics. Methods Mol Biol. 2011, 686: 297-311. 10.1007/978-1-60761-938-3_14.PubMedView ArticleGoogle Scholar
- Ma L, Mauro C, Cornish GH, Chai JG, Coe D, Fu H, Patton D, Okkenhaug K, Franzoso G, Dyson J, Nourshargh S, Marelli-Berg FM: Ig gene-like molecule CD31 plays a nonredundant role in the regulation of T-cell immunity and tolerance. Proc Natl Acad Sci U S A. 2010, 107: 19461-19466. 10.1073/pnas.1011748107.PubMedPubMed CentralView ArticleGoogle Scholar
- Anandasabapathy N, Victora GD, Meredith M, Feder R, Dong B, Kluger C, Yao K, Dustin ML, Nussenzweig MC, Steinman RM, Liu K: Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain. J Exp Med. 2011, 208: 1695-1705. 10.1084/jem.20102657.PubMedPubMed CentralView ArticleGoogle Scholar
- Engelhardt B: Molecular mechanisms involved in T cell migration across the blood–brain barrier. J Neural Transm. 2006, 113: 477-485. 10.1007/s00702-005-0409-y.PubMedView ArticleGoogle Scholar
- dos Santos AC, Barsante MM, Arantes RM, Bernard CC, Teixeira MM, Carvalho-Tavares J: CCL2 and CCL5 mediate leukocyte adhesion in experimental autoimmune encephalomyelitis–an intravital microscopy study. J Neuroimmunol. 2005, 162: 122-129. 10.1016/j.jneuroim.2005.01.020.PubMedView ArticleGoogle Scholar
- Moon C, Ahn M, Wie MB, Kim HM, Koh CS, Hong SC, Kim MD, Tanuma N, Matsumoto Y, Shin T: Phenidone, a dual inhibitor of cyclooxygenases and lipoxygenases, ameliorates rat paralysis in experimental autoimmune encephalomyelitis by suppressing its target enzymes. Brain Res. 2005, 1035: 206-210. 10.1016/j.brainres.2004.12.017.PubMedView ArticleGoogle Scholar
- Perez-Nievas BG, Garcia-Bueno B, Madrigal JL, Leza JC: Chronic immobilisation stress ameliorates clinical score and neuroinflammation in a MOG-induced EAE in Dark Agouti rats: mechanisms implicated. J Neuroinflammation. 2010, 7: 60-10.1186/1742-2094-7-60.PubMedPubMed CentralView ArticleGoogle Scholar
- Chitnis T, Najafian N, Benou C, Salama AD, Grusby MJ, Sayegh MH, Khoury SJ: Effect of targeted disruption of STAT4 and STAT6 on the induction of experimental autoimmune encephalomyelitis. J Clin Invest. 2001, 108: 739-747.PubMedPubMed CentralView ArticleGoogle Scholar
- Elgueta R, Benson MJ, de Vries VC, Wasiuk A, Guo Y, Noelle RJ: Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol Rev. 2009, 229: 152-172. 10.1111/j.1600-065X.2009.00782.x.PubMedView ArticleGoogle Scholar
- Bethea M, Forman DT: Beta 2-microglobulin: its significance and clinical usefulness. Ann Clin Lab Sci. 1990, 20: 163-168.PubMedGoogle Scholar
- Huang DR, Wang J, Kivisakk P, Rollins BJ, Ransohoff RM: Absence of monocyte chemoattractant protein 1 in mice leads to decreased local macrophage recruitment and antigen-specific T helper cell type 1 immune response in experimental autoimmune encephalomyelitis. J Exp Med. 2001, 193: 713-726. 10.1084/jem.193.6.713.PubMedPubMed CentralView ArticleGoogle Scholar
- Dogan RN, Elhofy A, Karpus WJ: Production of CCL2 by central nervous system cells regulates development of murine experimental autoimmune encephalomyelitis through the recruitment of TNF- and iNOS-expressing macrophages and myeloid dendritic cells. J Immunol. 2008, 180: 7376-7384.PubMedView ArticleGoogle Scholar
- Lee JM, Olitsky PK: Simple method for enhancing development of acute disseminated encephalomyelitis in mice. Proc Soc Exp Biol Med. 1955, 89: 263-266.PubMedView ArticleGoogle Scholar
- Levine S, Sowinski R: Experimental allergic encephalomyelitis in inbred and outbred mice. J Immunol. 1973, 110: 139-143.PubMedGoogle Scholar
- Mochizuki M, Charley J, Kuwabara T, Nussenblatt RB, Gery I: Involvement of the pineal gland in rats with experimental autoimmune uveitis. Invest Ophthalmol Vis Sci. 1983, 24: 1333-1338.PubMedGoogle Scholar
- Tung K, Taguchi O, Tester C: Testicular and ovarian autoimmune diseases. Autoimmune Disease Models. Edited by: Cohen I, Miller A. 1994, Academic, San Diego, 267-290.Google Scholar
- Caspi RR, Silver PB, Chan CC, Sun B, Agarwal RK, Wells J, Oddo S, Fujino Y, Najafian F, Wilder RL: Genetic susceptibility to experimental autoimmune uveoretinitis in the rat is associated with an elevated Th1 response. J Immunol. 1996, 157: 2668-2675.PubMedGoogle Scholar
- Garcia JG, Wang P, Liu F, Hershenson MB, Borbiev T, Verin AD: Pertussis toxin directly activates endothelial cell p42/p44 MAP kinases via a novel signaling pathway. Am J Physiol Cell Physiol. 2001, 280: C1233-C1241.PubMedGoogle Scholar
- Garcia JG, Wang P, Schaphorst KL, Becker PM, Borbiev T, Liu F, Birukova A, Jacobs K, Bogatcheva N, Verin AD: Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability. FASEB J. 2002, 16: 1064-1076. 10.1096/fj.01-0895com.PubMedView ArticleGoogle Scholar
- Munoz J: Action of pertussigen (pertussis toxin) on the host immune system. Pathogenesis and Immunity in Pertussis. Edited by: Wardlaw AC, Partoin R. 1988, Wiley, New York, 173-187.Google Scholar
- Linthicum DS, Munoz JJ, Blaskett A: Acute experimental autoimmune encephalomyelitis in mice. I. Adjuvant action of Bordetella pertussis is due to vasoactive amine sensitization and increased vascular permeability of the central nervous system. Cell Immunol. 1982, 73: 299-310. 10.1016/0008-8749(82)90457-9.PubMedView ArticleGoogle Scholar
- Yong T, Meininger GA, Linthicum DS: Enhancement of histamine-induced vascular leakage by pertussis toxin in SJL/J mice but not BALB/c mice. J Neuroimmunol. 1993, 45: 47-52. 10.1016/0165-5728(93)90162-R.PubMedView ArticleGoogle Scholar
- Kugler S, Bocker K, Heusipp G, Greune L, Kim KS, Schmidt MA: Pertussis toxin transiently affects barrier integrity, organelle organization and transmigration of monocytes in a human brain microvascular endothelial cell barrier model. Cell Microbiol. 2007, 9: 619-632. 10.1111/j.1462-5822.2006.00813.x.PubMedView ArticleGoogle Scholar
- Bruckener KE, el Baya A, Galla HJ, Schmidt MA: Permeabilization in a cerebral endothelial barrier model by pertussis toxin involves the PKC effector pathway and is abolished by elevated levels of cAMP. J Cell Sci. 2003, 116: 1837-1846. 10.1242/jcs.00378.PubMedView ArticleGoogle Scholar
- Blankenhorn EP, Butterfield RJ, Rigby R, Cort L, Giambrone D, McDermott P, McEntee K, Solowski N, Meeker ND, Zachary JF, Doerge RW, Teuscher C: Genetic analysis of the influence of pertussis toxin on experimental allergic encephalomyelitis susceptibility: an environmental agent can override genetic checkpoints. J Immunol. 2000, 164: 3420-3425.PubMedView ArticleGoogle Scholar
- Hofstetter HH, Shive CL, Forsthuber TG: Pertussis toxin modulates the immune response to neuroantigens injected in incomplete Freund's adjuvant: induction of Th1 cells and experimental autoimmune encephalomyelitis in the presence of high frequencies of Th2 cells. J Immunol. 2002, 169: 117-125.PubMedView ArticleGoogle Scholar
- Racke MK, Hu W, Lovett-Racke AE: PTX cruiser: driving autoimmunity via TLR4. Trends Immunol. 2005, 26: 289-291. 10.1016/j.it.2005.03.012.PubMedView ArticleGoogle Scholar
- Richard JF, Roy M, Audoy-Remus J, Tremblay P, Vallieres L: Crawling phagocytes recruited in the brain vasculature after pertussis toxin exposure through IL6, ICAM1 and ITGalphaM. Brain Pathol. 2011, 21: 661-671. 10.1111/j.1750-3639.2011.00490.x.PubMedView ArticleGoogle Scholar
- Maharaj AS, Walshe TE, Saint-Geniez M, Venkatesha S, Maldonado AE, Himes NC, Matharu KS, Karumanchi SA, D'Amore PA: VEGF and TGF-beta are required for the maintenance of the choroid plexus and ependyma. J Exp Med. 2008, 205: 491-501. 10.1084/jem.20072041.PubMedPubMed CentralView ArticleGoogle Scholar
- Frank PG, Pavlides S, Lisanti MP: Caveolae and transcytosis in endothelial cells: role in atherosclerosis. Cell Tissue Res. 2009, 335: 41-47. 10.1007/s00441-008-0659-8.PubMedView ArticleGoogle Scholar
- Ge S, Song L, Serwanski DR, Kuziel WA, Pachter JS: Transcellular transport of CCL2 across brain microvascular endothelial cells. J Neurochem. 2008, 104: 1219-1232. 10.1111/j.1471-4159.2007.05056.x.PubMedView ArticleGoogle Scholar
- Chidlow JH, Sessa WC: Caveolae, caveolins, and cavins: complex control of cellular signalling and inflammation. Cardiovasc Res. 2010, 86: 219-225. 10.1093/cvr/cvq075.PubMedPubMed CentralView ArticleGoogle Scholar
- Komarova Y, Malik AB: Regulation of endothelial permeability via paracellular and transcellular transport pathways. Annu Rev Physiol. 2010, 72: 463-493. 10.1146/annurev-physiol-021909-135833.PubMedView ArticleGoogle Scholar
- Brabb T, Goldrath AW, von Dassow P, Paez A, Liggitt HD, Goverman J: Triggers of autoimmune disease in a murine TCR-transgenic model for multiple sclerosis. J Immunol. 1997, 159: 497-507.PubMedGoogle Scholar
- Mitchell K, Yang HY, Berk JD, Tran JH, Iadarola MJ: Monocyte chemoattractant protein-1 in the choroid plexus: a potential link between vascular pro-inflammatory mediators and the CNS during peripheral tissue inflammation. Neuroscience. 2009, 158: 885-895. 10.1016/j.neuroscience.2008.10.047.PubMedPubMed CentralView ArticleGoogle Scholar
- Schellenberg AE, Buist R, Del Bigio MR, Khorooshi R, Toft-Hansen H, Owens T, Peeling J: Blood–brain barrier disruption in CCL2 transgenic mice during pertussis toxin-induced brain inflammation. Fluids Barriers CNS. 2012, 9: 10-10.1186/2045-8118-9-10.PubMedPubMed CentralView ArticleGoogle Scholar
- Goverman J, Woods A, Larson L, Weiner LP, Hood L, Zaller DM: Transgenic mice that express a myelin basic protein-specific T cell receptor develop spontaneous autoimmunity. Cell. 1993, 72: 551-560. 10.1016/0092-8674(93)90074-Z.PubMedView ArticleGoogle Scholar
- Murphey C, Chang S, Zhang X, Arulanandam B, Forsthuber TG: Induction of polyclonal CD8+ T cell activation and effector function by Pertussis toxin. Cell Immunol. 2011, 267: 50-55. 10.1016/j.cellimm.2010.11.003.PubMedPubMed CentralView ArticleGoogle Scholar
- Kuwabara T, Ishikawa F, Yasuda T, Aritomi K, Nakano H, Tanaka Y, Okada Y, Lipp M, Kakiuchi T: CCR7 ligands are required for development of experimental autoimmune encephalomyelitis through generating IL-23-dependent Th17 cells. J Immunol. 2009, 183: 2513-2521. 10.4049/jimmunol.0800729.PubMedView ArticleGoogle Scholar
- Szmydynger-Chodobska J, Strazielle N, Gandy JR, Keefe TH, Zink BJ, Ghersi-Egea JF, Chodobski A: Posttraumatic invasion of monocytes across the blood-cerebrospinal fluid barrier. J Cereb Blood Flow Metab. 2012, 32: 93-104. 10.1038/jcbfm.2011.111.PubMedPubMed CentralView ArticleGoogle Scholar
- Monzon ME, Forteza RM, Casalino-Matsuda SM: MCP-1/CCR2B-dependent loop upregulates MUC5AC and MUC5B in human airway epithelium. Am J Physiol Lung Cell Mol Physiol. 2011, 300: L204-L215. 10.1152/ajplung.00292.2010.PubMedPubMed CentralView ArticleGoogle Scholar
- van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P: Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis. Clin Exp Immunol. 2009, 158: 143-153. 10.1111/j.1365-2249.2009.03993.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Muzio L, Cavasinni F, Marinaro C, Bergamaschi A, Bergami A, Porcheri C, Cerri F, Dina G, Quattrini A, Comi G, Furlan R, Martino G: Cxcl10 enhances blood cells migration in the sub-ventricular zone of mice affected by experimental autoimmune encephalomyelitis. Mol Cell Neurosci. 2010, 43: 268-280. 10.1016/j.mcn.2009.11.008.PubMedView ArticleGoogle Scholar
- Marques F, Sousa JC, Coppola G, Geschwind DH, Sousa N, Palha JA, Correia-Neves M: The choroid plexus response to a repeated peripheral inflammatory stimulus. BMC Neurosci. 2009, 10: 135-10.1186/1471-2202-10-135.PubMedPubMed CentralView ArticleGoogle Scholar
- Freria CM, Zanon RG, Santos LM, Oliveira AL: Major histocompatibility complex class I expression and glial reaction influence spinal motoneuron synaptic plasticity during the course of experimental autoimmune encephalomyelitis. J Comp Neurol. 2010, 518: 990-1007. 10.1002/cne.22259.PubMedView ArticleGoogle Scholar
- Jain MR, Bian S, Liu T, Hu J, Elkabes S, Li H: Altered proteolytic events in experimental autoimmune encephalomyelitis discovered by iTRAQ shotgun proteomics analysis of spinal cord. Proteome Sci. 2009, 7: 25-10.1186/1477-5956-7-25.PubMedPubMed CentralView ArticleGoogle Scholar
- Szalai AJ, Hu X, Adams JE, Barnum SR: Complement in experimental autoimmune encephalomyelitis revisited: C3 is required for development of maximal disease. Mol Immunol. 2007, 44: 3132-3136. 10.1016/j.molimm.2007.02.002.PubMedPubMed CentralView ArticleGoogle Scholar
- Engelhardt B, Sorokin L: The blood–brain and the blood-cerebrospinal fluid barriers: function and dysfunction. Semin Immunopathol. 2009, 31: 497-511. 10.1007/s00281-009-0177-0.PubMedView ArticleGoogle Scholar
- Liddelow SA, Temple S, Mollgard K, Gehwolf R, Wagner A, Bauer H, Bauer HC, Phoenix TN, Dziegielewska KM, Saunders NR: Molecular characterisation of transport mechanisms at the developing mouse blood-CSF interface: a transcriptome approach. PLoS One. 2012, 7: e33554-10.1371/journal.pone.0033554.PubMedPubMed CentralView ArticleGoogle Scholar
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