Fig. 5

Brain ZO-1, claudin-5, and occludin expression in mice after 14 days of JUUL/TS exposure with or without MCAO. A Western blot images of ZO-1 expression in normoxic brain and contralateral and ipsilateral brain hemispheres 24 h after MCAO. B–D Quantification of brain ZO-1 expression normalized to beta-actin and expressed as relative to control (1.0) in normoxic, contralateral, and ipsilateral brain regions. E Western blot images of claudin-5 expression in normoxic brain and contralateral and ipsilateral brain hemispheres 24 h after MCAO. F–H Quantification of brain claudin-5 expression normalized to beta-actin and expressed as relative to control (1.0) in normoxic, contralateral, and ipsilateral brain regions, respectively. I Western blot images of occludin expression in normoxic brain and contralateral & ipsilateral brain hemispheres 24 h after MCAO. J–L Quantification of brain occludin expression normalized to beta-actin and expressed as relative to control (1.0) in normoxic, contralateral, and ipsilateral brain regions. *P < 0.05, ***P < 0.001; n = 8 for each group (B), n = 6, 6, and 5 for control, JUUL, and TS respectively (C), n = 7, 7, and 6 for control, JUUL, and TS respectively (D), n = 9 for each group (F), n = 8, 7, and 6 for control, JUUL, and TS respectively (G), n = 8, 8, and 6 for control, JUUL, and TS respectively (H), n = 9 for each group (J), n = 7, 6, and 5 for control, JUUL, and TS respectively (K), n = 6 for each group (L). Cropped images from blots have been used in some cases to improve the clarity and conciseness of the presentation. The cropped images are delineated with white space; full-length blots developed by X-ray films are presented in Additional file 1: Fig. S1