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Figure 1 | Fluids and Barriers of the CNS

Figure 1

From: Attenuation of prostaglandin E2 elimination across the mouse blood-brain barrier in lipopolysaccharide-induced inflammation and additive inhibitory effect of cefmetazole

Figure 1

Effect of LPS treatment on elimination of [3H]PGE 2 from mouse brain. (A) Time-course of [3H]PGE2 in the ipsilateral cerebrum after intracerebral microinjection in LPS-treated mice (open circles) and saline-treated mice (closed circles). A mixture of [3H]PGE2 (96 nCi) and [14C]inulin (4.8 nCi; internal reference) dissolved in 0.3 μL ECF buffer containing 0.1% ethanol was injected into the S2 region of mouse brain. Each point represents the mean ± SEM (n = 4 - 5). **p < 0.01, significantly different from the saline-treated mice. (B) Effect of unlabeled PGE2 on the percentage of [3H]PGE2 remaining in the ipsilateral cerebrum at 40 min after intracerebral microinjection in LPS-treated mice. A mixture of [3H]PGE2 and [14C]inulin dissolved in 0.3 μL ECF buffer containing 3 mM PGE2 was injected. Each column represents the mean ± S.E.M. (n = 3 - 4). *p < 0.05, significantly different from the tracer only.

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Fluids and Barriers of the CNS

ISSN: 2045-8118