Figure 1From: Resolution of central nervous system astrocytic and endothelial sources of CCL2 gene expression during evolving neuroinflammationCCL2 gene expression in CNS microvessel and parenchymal fractions obtained by bulk preparation. Separate, crude microvessel (A, C) and parenchymal (B, D) fractions were prepared by bulk isolation from combined brain and spinal cord tissue of mice injected with CFA/PTX (A, B) or immunized with MOG-CFA/PTX (C, D). Analyses were performed at D9, D16 and D23 following beginning of either injection regime, and relative CCL2 mRNA levels are plotted on a log scale. Comparisons were made to naïve mice. *P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001. Data has been presented as mean +/− SEM. For each experiment, bulk isolations were performed from three individual mice from each naïve, control (CFA/PTX) and EAE (MOG-CFA/PTX) group, and experiments were conducted twice.Back to article page