Quantitative imaging assessment of blood-brain barrier permeability in humans
© Chassidim et al.; licensee BioMed Central Ltd. 2013
Received: 3 October 2012
Accepted: 4 February 2013
Published: 7 February 2013
The blood–brain barrier (BBB) is a functional and structural barrier separating the intravascular and neuropil compartments of the brain. It characterizes the vascular bed and is essential for normal brain functions. Dysfunction in the BBB properties have been described in most common neurological disorders, such as stroke, traumatic injuries, intracerebral hemorrhage, tumors, epilepsy and neurodegenerative disorders. It is now obvious that the BBB plays an important role in normal brain activity, stressing the need for applicable imaging and assessment methods. Recent advancements in imaging techniques now make it possible to establish sensitive and quantitative methods for the assessment of BBB permeability. However, most of the existing techniques require complicated and demanding dynamic scanning protocols that are impractical and cannot be fulfilled in some cases. We review existing methods for the evaluation of BBB permeability, focusing on quantitative magnetic resonance-based approaches and discuss their drawbacks and limitations. In light of those limitations we propose two new approaches for BBB assessment with less demanding imaging sequences: the “post-pre” and the “linear dynamic” methods, both allow semi-quantitative permeability assessment and localization of dysfunctional BBB with simple/partial dynamic imaging protocols and easy-to-apply analysis algorithms. We present preliminary results and show an example which compares these new methods with the existing standard assessment method. We strongly believe that the establishment of such “easy to use” and reliable imaging methods is essential before BBB assessment can become a routine clinical tool. Large clinical trials are awaited to fully understand the significance of BBB permeability as a biomarker and target for treatment in neurological disorders.
The blood–brain barrier (BBB) is a tightly-regulated, structural and functional barrier that controls the extracellular neuronal environment within the brain and spinal cord by limiting the free passage of ions and large molecules into the CNS. BBB functions are often impaired in common neurological disorders, including stroke, traumatic injuries, intracerebral hemorrhage, tumors, epilepsy and neurodegenerative disorders. BBB dysfunction is often associated with increased vascular permeability to plasma constituents, including large proteins, and results in water influx and brain edema . Recent animal experiments demonstrated that serum proteins may serve as direct signaling mechanism within the brain resulting in the activation of astrocytes and the brain immune system, with a consequent neuronal hyperexcitability and delayed neurodegeneration . These studies have highlighted BBB dysfunction as a potential biomarker for neurological disorders, with the possibility for predicting complications and neurological outcome after an insult and for determining novel treatments. Recent advancements in imaging methods now make it possible to establish sensitive and quantitative methods for the assessment of BBB permeability, most of which are MRI based.
Methods based on magnetic resonance imaging (MRI) scans following intravenous injection of MR-visible contrast agents containing gadolinium are currently the gold standard and most commonly used non-invasive imaging technique for the assessment of BBB impairment in both clinical [3–5] and preclinical studies . One of the main advantages of MRI is the ability to produce multi-parametric information, allowing high-resolution anatomical information, and assessment of cerebral blood flow (CBF) and BBB integrity using the same imaging modality . BBB assessment and permeability quantification are carried out by either using semi-quantitative methods, where statistical differences between scans before and after tracer injection are calculated , or by using more quantitative approaches based on a dynamic contrast enhanced MRI (DCE-MRI) [9, 10]. These approaches assess the kinetics of a contrast agent over time and space to detect regions with increased vessel permeability. However, most of the existing techniques require complicated and demanding dynamic scanning protocols, including tracer injection during the scan and long scanning sessions. These imaging protocols in addition to the complex and computationally-demanding assessment algorithms, make these dynamic methods impractical and in some cases even unfeasible, stressing the need for developing “lighter” and less demanding semi-quantitative methods. One limitation occurs particularly in cases where patients need to be scanned immediately following a treatment that cannot be performed inside the magnet, preventing tracer injection during the scanning as required in DCE-MRI. We describe and discuss two new methods with less demanding MRI protocols and easy-to-apply analysis algorithms, the post-pre and linear dynamic methods. In the post-pre method only two scans are statistically compared and analyzed, one scan before the tracer injection and the other after the tracer injection. Regions with statistically-significant positive changes are marked as potentially permeable and further processed. The linear dynamic method is a semi-dynamic approach, where scans are taken at several time points after the tracer injection and analyzed according to their linear slope. Both proposed methods overcome some of the limitations described while supplying semi-quantitative assessment comparable to the existing more complex method.
The post-pre approach is based on a comparative analysis between contrast-enhanced MR scans before and after the intravenous contrast agent injection. Scans acquired after the injection show regions with enhanced contrast agent, which can be quantified by comparison with the corresponding pre-injection non-contrast scans. Here we present an advanced method for permeability assessment based on statistical analysis of the imaging.
T1-weighted sequences are taken before and 5 minutes after the injection of Gd-DTPA with dose normalized according to patient’s body weight (Gd-DTPA 0.5M, 0.1mmol/kg). The scans are mapped to a standard brain atlas to avoid artifacts due to head movements and to allow accurate pixel based comparison between scans and even between patients for group studies analysis. This can also be achieved by co-registration between the pre- and post-injection images. Regions outside the brain are excluded from the images and the brain region is segmented into three tissue classes: gray matter, white matter and cerebrospinal fluid (CSF). A brain tissue segment is defined by the combination of the gray and white matter regions.
Pre- and post- contrast images are compared in several steps. First, significantly different pixels are identified by performing a slice-wise unpaired t-test (slice-wise processing was chosen for images with non-isometric voxel size). To this goal, a neighborhood comparison is applied where for each pixel, the 3x3 neighborhood (nine pixels) in the pre- and post- contrast images are compared. To avoid over-estimation of the significance level due to redundant t-tests of each pixel, the FDR (false discovery rate) statistical correction is applied . Each pixel in the image is assigned the significance level accordingly, resulting in a statistical significance image (P-values). Pixels with P-values < 0.05 are indicated as statistically significant, resulting in a binary significance image, where all significant pixels are assigned with 1 and the non-significant pixels with 0. The second step involved a calculation of the enhancement differences between the pre and post contrast images, which is computed for each pixel and referred to as E (percentage value of the intensity difference).
The enhancement-distribution histograms of three representing regions: eyeball, muscle and a blood vessel are calculated in order to determine BBB breakdown enhancement range for each individual subject (Figure 1). Muscle is used as a normalization reference to determine intensity for a “permeable tissue” with no BBB, and the blood vessel region used to exclude pixels which represent vessels. Thus, a pixel is considered as a “permeable brain region” when its intensity significantly changed after contrast injection and it was in the range between the intact BBB (“eyeball”) and the blood vessel. In order to define an enhancement range, a Gaussian fit is applied to the histogram that makes it possible to estimate the mean μ and standard deviation δ of the enhancement distribution. Significantly different pixels (taken from the binary significant image) within the enhancement range of μ ±3δ are considered to represent brain tissue with leaky BBB (denoted as potential for leaky BBB, PBBB).
The third step applies a clustering procedure on the potentially permeable pixels. Neighboring pixels are aggregated into anatomically-connected regions (Figure 2E) in order to establish a more robust assessment of the clinically-relevant brain regions with a pathological BBB. This was achieved mathematically by applying image processing operations which rendered closely-located pixels into clusters, followed by component labeling to select pixel clusters with cluster areas larger than a minimal area. For this purpose, all the objects inside the image were ranked according to their area and connectivity (4-connected). Clusters with areas smaller than the minimal one are removed from the image. As a result, regions with a high density of permeable pixels are highlighted, while single pixels are removed as potential noise artifacts.
The “linear dynamic method”
In this approach a dynamic study is performed. There are situations that require the contrast agent to be injected before the patient enters the scanner. The time-gap between injection and the beginning of the scan can be up to several minutes (e.g., following a trans-cranial magnetic stimulation). Under such conditions, the standard two-compartment model cannot be used, since the arterial input function (AIF) cannot be estimated. Quantitative analysis can be carried out by fitting a linear curve to the dynamic scan intensities. To allow inter-scan comparisons, the time between the injection and the scan should be similar. That is, a signal s (t) is fitted to a linear curve: s(t) = A · t + B. The slope (A) can be interpreted as the rate of wash-in or wash-out of the contrast agent, whereas the intercept (B) represents the relative amount of contrast agent at the beginning of scanning. A “goodness of fit” map (R2) can also be created. Areas with different anatomical properties are hypothesized to demonstrate different parameters (i.e. blood vessels should display a relatively high intercept and a negative slope with a large absolute value; while brain with an intact BBB is expected to display a low intercept and a “close to zero” slope).
The ability to use this parametric map for both inter- and intra-subject comparison is an important goal. However it is not straightforward: firstly due to the inherent non-linearity of the MR signal intensity with respect to contrast agent concentration, which makes it impossible to compare different scans quantitatively. Secondly, variability in the administration of the contrast agent and different physiological states of the subject, affect the reproducibility of such scans.
Thus, data normalization should be considered to overcome these issues. We propose two methods which use the selection of a region of interest (ROI) as a reference. The signal in the selected ROI is then averaged and used in one of two ways:
Parametric normalization: A linear curve is fitted to the averaged signal of the selected ROI, producing slope (SLROI) and intercept (INROI).
Temporal normalization: The temporal signal in each voxel is normalized to the corresponding signal in the ROI. A linear curve is then fitted to the normalized signal.
Discussion and conclusion
In this manuscript we described previous and new attempts to assess BBB permeability quantitatively in humans. While the DCE-MRI has been described for more than a decade and shown to yield dysfunctional BBB under different clinical conditions, it has not been implemented in the routine clinical setup, nor tested for its power in predicting patients’ outcome, or having any contribution to clinical decision. A major limitation of DCE-MRI is the complex imaging requirements, which include initiation of the dynamic sequence prior to the intravenous injection within the magnet, followed by high frequency repeated scanning, that may force lower spatial resolution or reduced coverage of brain regions. The high temporal resolution is mainly needed for sampling the rapid passage of tracer in the arterial phase, which occurs within seconds following the injection. Although highly informative, in particular for CBF assessment, the first bolus pass of the contrast agent may be neglected while retaining sufficient information in the later stages after injection for BBB integrity assessment. A dual-temporal resolution imaging protocol has been proposed , based on an initial sequence with a high acquisition rate to sample the first arterial pass followed by a slower acquisition rate to sample the rest of the signal curve for a longer time. Although partially overcoming the demanding high frequency requirement, it is still based on the arterial input function and therefore tracer injection must be done during scanning. This requirement possesses complexity, which cannot always be overcome in the clinical setup, due to patient care or treatment conditions or even on-site equipment and hardware limitations. Another limitation is the low spatial resolution and the limited brain volume required when high acquisition rate is implemented. A further issue is the computation resources needed for permeability analysis of DCE-MRI, particularly if real-time analysis is desired. A full brain DCE-MRI permeability analysis can take up to an hour processing in a standard work station. The proposed new methods presented in this paper may provide a non-compromising alternative to the full dynamic methods with a much less demanding imaging protocol and computation time. In even more restricting scenarios, when the patient is unable to stay in the MR magnet for long enough periods, the post-pre semi-quantitative method may serve as an option for BBB assessment. However, it is not as yet clear whether these methods may reflect different levels of BBB dysfunction. Another issue is the lack of comparison studies between the methodologies, their sensitivity, or their ability to predict patient outcome.
Quantitative analysis using DCE-MRI
In most cases, the pharmacokinetic models used for quantification of permeability from contrast-enhanced data are based on those developed for nuclear medicine. These models are based on the two-compartment exchange model (2CXM)  in which the contrast agent is accessible to two compartments within the overall tissue volume (“voxel”) comprising blood (“intravascular”) and tissue (“extravascular”) compartments. The latter is usually divided into the extracellular- extravascular space (EES) and the intracellular space. A common assumption is that the contrast agent is impermeable to cell boundaries so that these blood and tissue compartments essentially represent plasma and EES spaces respectively. The contrast agent is partitioned between these two compartments with concentrations of Cp(t) and Ct(t), respectively. The goal of contrast-enhanced techniques for permeability quantification is to measure these two dynamic quantities and thereby derive permeability-related measures according to the appropriate pharmacokinetic model. Under normal circumstances, the contrast agent is confined to the intravascular space and its passage through the volume is controlled by blood flow. However, when BBB functions are impaired, leakage will occur into the tissue (EES) compartment. This depends not only on chemical characteristics of the contrast agent, but also on the physiological characteristics of the interface between the two compartments, i.e., the BBB. Under conditions of BBB breakdown or impaired vascularity, the contrast agent can escape the vasculature and accumulate in the interstitial space of the tissue (“extravasation”) and the concentration of tracer in the tissue compartment is correspondingly increased.
As passage of the contrast agent through the blood and into the tissue reflects the competing influences of vascular perfusion and leakage, the 2CXM model enables quantification of the capillary permeability-surface area product, PS, and tissue plasma perfusion, Fp. The “standard Tofts model” [14, 15] is a simplification of this two compartment model that assumes a negligible contribution of the plasma space and therefore essentially represents a one-compartment model. The “modified Tofts model” reinstates the contribution of the plasma compartment but makes a simplifying assumption that the time taken for the contrast agent to pass through this compartment (the plasma mean transit time, MTT) is negligible .
where ktrans, known as the transfer constant, defines the rate of transfer from plasma to EES compartments in (mL/g/min), and ve, known as the extracellular space fraction, describes the fraction of the extravascular-extracellular space occupied by tracer in (mL/g). ktrans incorporates in its formulation both PS and Fp so that these terms cannot be dissociated. By relating to Eq. (1), it can be seen that the IRF is formulated as R Tofts (t) = (ktrans/Fp). exp(−tktrans/Ve) :
where vp is the fraction of the tissue volume occupied by blood plasma [mL/g].
Derivation of hemodynamic parameters from the concentration-time curves based on these models follow the application of principles from tracer-kinetic analysis . Approaches follow either direct methods that derive some of the parameters without explicitly determining the IRF, or deconvolution methods that derive the full IRF via model-driven, model-free or parametric approaches.
The MRI approach to contrast-enhanced permeability quantification (principally DCE-MRI) calculates the concentration-time curves, C(t), of a MR-visible contrast agent from the dynamic MRI signal intensity, S(t), according to MR principles. Gd-DTPA, a freely diffusible, extracellular tracer, is the most common contrast agent in these studies whose paramagnetic Gd3+ core reduces the relaxation time, T1, in a concentration-dependent manner. A set of T1-weighted images is acquired, starting before a short bolus injection, and continued as uptake by the tissue followed by washout occurs. The presence of the contrast agent is indicated by an increase in signal intensity. The quantification approach relies on the assumption of a linear relationship between the T1 relaxation rate (R1 = 1/T1) and the contrast agent concentration which is believed to be valid for the relatively low concentrations expected in standard DCE-MRI studies. The most common MRI pulse sequence used in these studies is a rapid 3D gradient echo sequence for which the relationship between the signal intensity and the R1 is well-defined but non-linear. The concentration-time curves can be calculated from the dynamically acquired images if a baseline measurement of the relaxation time is performed before bolus injection. For quantitative analysis, an accurate measure of the AIF is also required. This can either be derived from a population-averaged experimental measurement  or directly measured in the subject within an imaging region containing a cerebral artery or within the drainage veins of the superior sagittal sinus.
- Shlosberg D, Benifla M, Kaufer D, Friedman A: Blood–brain barrier breakdown as a therapeutic target in traumatic brain injury. Nat Rev Neurol. 2010, 6: 393-403. 10.1038/nrneurol.2010.74.PubMed CentralPubMedView ArticleGoogle Scholar
- Abbot NJ, Friedman A: Overview and introduction: The blood–brain barrier in health and disease. Epilepsia. 2012, 53 (Suppl. 6): 1-6.View ArticleGoogle Scholar
- Tomkins O, Shelef I, Kaizerman I, Eliushin A, Afawi Z, Misk A, Gidon M, Cohen A, Zumsteg D, Friedman A: Blood–brain barrier disruption in post-traumatic epilepsy. J Neurol Neurosurg Psychiat. 2008, 79: 774-777. 10.1136/jnnp.2007.126425.PubMedView ArticleGoogle Scholar
- Tomkins O, Feintuch A, Benifla M, Cohen A, Friedman A, Shelef I: Blood–brain barrier breakdown following traumatic brain injury: a possible role in posttraumatic epilepsy. Cardiovasc Psychiatry Neurol. 2011, 765923: 1-11.View ArticleGoogle Scholar
- Schoknecht K, Shalev H: Blood–brain barrier dysfunction in brain diseases: Clinical experience. Epilepsia. 2012, 53 (Suppl. 6): 7-13.PubMedView ArticleGoogle Scholar
- Rebeles F, Fink J, Anzai Y, Maravilla KR: Blood–brain barrier imaging and therapeutic potentials. Top Magn Reson Imaging. 2006, 17: 107-116. 10.1097/RMR.0b013e31802f5df9.PubMedView ArticleGoogle Scholar
- Nagaraja TN, Knight RA, Ewing JR, Karki K, Nagesh V, Fenstermacher JD: Multiparametric Magnetic Resonance Imaging and Repeated Measurements of Blood–brain Barrier Permeability to Contrast Agents. The blood–brain and other neural barriers. Edited by: Sukriti N. 2011, New York: Humana Press, 193-212.View ArticleGoogle Scholar
- Schellenberg AE, Buist R, Yong VW, Del Bigio MR, Peeling J: Magnetic resonance imaging of blood-spinal cord barrier disruption in mice with experimental autoimmune encephalomyelitis. J Magn Reson Med. 2007, 58: 298-305. 10.1002/mrm.21289.View ArticleGoogle Scholar
- Knight RA, Nagaraja TN, Ewing JR, Nagesh V, Whitton PA, Bershad E, Fagan SC, Fenstermacher JD: Quantitation and localization of blood-to-brain influx by magnetic resonance imaging and quantitative autoradiography in a model of transient focal ischemia. J Magn Reson Med. 2005, 54: 813-821. 10.1002/mrm.20629.View ArticleGoogle Scholar
- Sourbron SP, Buckley DL: Tracer kinetic modelling in MRI: estimating perfusion and capillary permeability. Phys Med Biol. 2012, 57: R1-R33. 10.1088/0031-9155/57/2/R1.PubMedView ArticleGoogle Scholar
- Benjamini Y: Discovering the false discovery rate. Journal of the Royal Statistical Society Series B. 2010, 72: 405-416. 10.1111/j.1467-9868.2010.00746.x.View ArticleGoogle Scholar
- Jelescu IO, Leppert IR, Narayanan S, Araújo D, Arnold DL, Pike GB: Dual-temporal resolution dynamic contrast-enhanced MRI protocol for blood–brain barrier permeability measurement in enhancing multiple sclerosis lesions. J Magn Reson Imaging. 2011, 33: 1291-1300. 10.1002/jmri.22565.PubMedView ArticleGoogle Scholar
- Brix G, Kiessling F, Lucht R, Darai S, Wasser K, Delorme S, Griebel J: Microcirculation and microvasculature in breast tumors: pharmacokinetic analysis of dynamic MR image series. J Magn Reson Med. 2004, 52: 420-429. 10.1002/mrm.20161.View ArticleGoogle Scholar
- Tofts PS, Kermode AG: Measurement of the blood–brain barrier permeability and leakage space using dynamic MR imaging-Fundamental concepts. J Magn Reson Med. 1991, 17: 357-367. 10.1002/mrm.1910170208.View ArticleGoogle Scholar
- Tofts PS, Brix G, Buckley DL: Estimating kinetic parameters from dynamic contrast-enhanced T1-weighted MRI of a diffusible tracer: standardized quantities and symbols. J Magn Reson Imaging. 1999, 10: 223-232. 10.1002/(SICI)1522-2586(199909)10:3<223::AID-JMRI2>3.0.CO;2-S.PubMedView ArticleGoogle Scholar
- Tofts PS: Modeling tracer kinetics in dynamic Gd-DTPA MR imaging. J Magn Reson Imaging. 1997, 7: 91-101. 10.1002/jmri.1880070113.PubMedView ArticleGoogle Scholar
- Sourbron S: Technical aspects of MR perfusion. Eur J Radiol. 2010, 76: 304-313. 10.1016/j.ejrad.2010.02.017.PubMedView ArticleGoogle Scholar
- Parker GJ, Roberts C, Macdonald A, Buonaccorsi GA, Cheung S, Buckley DL, Jackson A, Watson Y, Davies K, Jayson GC: Experimentally-derived functional form for a population-averaged high-temporal-resolution arterial input function for dynamic contrast-enhanced MRI. J Magn Reson Med. 2006, 56: 993-1000. 10.1002/mrm.21066.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.