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Table 1 Proteins identified in HBEC-EMVs by proteomics that were in common with known markers of exosomes*

From: Method for isolation and molecular characterization of extracellular microvesicles released from brain endothelial cells

Protein category Gene symbol No. of Families detected
(1) Antigen-presentation   
HLA class I histocompatibility antigen HLA 2
(2) Cell adhesion   
Lactadherin MFGE8 1
Thromospondin-1 THBS1 1
Integrins ITG 5
(3) Cell structure and motility   
Actins ACT 12
α-Actinin-4 ACTN 2
Cofilin-1 CFL1 1
Moesin MSN 1
Myosin, heavy MYH 5
Myosin, light MYL 3
Radixin RDX 1
Tublins TUB 13
(4) Heat shock proteins and chaperones   
Heat shock cognate 71 kDa protein HSPA8 1
T-complex protein 1 CCT 8
(5) Metabolic enzymes   
Aldolase A ALDOA 1
Fatty acid synthase FASN 1
Glyceraldehyde-3-phosphate dehydrogenase GAPDH 1
Phosphoglycerate kinase 1 PGK1 1
Phosphoglycerate mutase 1 PGAM1 1
Pyruvate kinase isozymes M1/M2 PKM2 1
(6) Multi vesicular body (MVB) biogenesis   
Alix PDCD6IP 1
ESCRT I complex   
Tumor susceptibility gene 101 protein TSG101 1
Vacuolar sorting protein 28 VPS28 1
Vacuolar protein sorting-associatedprotein 37 VPS37 3
ESCRT II complex   
Vacuolar protein-sorting-associated protein 25 VPS25 1
Vacuolar protein-sorting-associated protein 36 VPS36 1
Vacuolar-sorting protein SNF8 SNF8 1
ESCRT III complex   
Charged MVB proteins CHMP 2
(7) Signaling proteins   
14-3-3 Proteins YWHA 6
GTPase HRas HRAS 1
Rho GDP-dissociation inhibitor 1 ARHGDIA 1
Rho-related GTP-binding protein RhoC precursor RHOC 1
Ras-related protein Rap-1b RAP1B 1
Ras-related protein R-Ras2 RRAS2 1
Ras GTPase-activating-like protein IQGAP1 1
Syntenin-1 SDCBP 1
Transforming protein RhoA RHOA 1
Guanine nucleotide-binding protein (G proteins)   
-G(I)/G(S)/G(T) subunit beta GNB 4
Protein category and description Gene symbol   
-G(I)/G(S)/G(O) subunit gamma GNG 1
-G(S) subunit alpha GNAS 1
-subunit alpha GNA 4
-G(I), alpha GNAI 1
(8) Tetraspanins   
CD9 antigen CD9 1
CD63 antigen CD63 1
CD81 antigen CD81 1
CD82 antigen CD82 1
(9) Transcription and protein synthesis   
Histones HIST 14
Ribosomal proteins RPS 34
Ubiquitin RPS27A 1
Elongation factor 1-a 1 EEF1A1 1
(10) Trafficking and membrane fusion   
Annexins ANXA 1
ADP-ribosylation factor ARF 3
AP-2 complex subunit α-1 AP2A1 1
AP-2 complex subunit β-1 AP2B1 1
Clathrin heavy chain 1 CLTC 1
Rab GDP dissociation inhibitor b GDI2 1
Ras-related protein Rab RAB 9
Synaptosomal-associated protein 23 SNAP23 1
  1. * The known markers of exosomes were obtained from Simpson et al[14]. For many proteins, more than one family member was detected.