Table 1 Proteins identified in HBEC-EMVs by proteomics that were in common with known markers of exosomes*
Protein category | Gene symbol | No. of Families detected |
|---|---|---|
(1) Antigen-presentation | ||
HLA class I histocompatibility antigen | HLA | 2 |
(2) Cell adhesion | ||
Lactadherin | MFGE8 | 1 |
Thromospondin-1 | THBS1 | 1 |
Integrins | ITG | 5 |
(3) Cell structure and motility | ||
Actins | ACT | 12 |
α-Actinin-4 | ACTN | 2 |
Cofilin-1 | CFL1 | 1 |
Moesin | MSN | 1 |
Myosin, heavy | MYH | 5 |
Myosin, light | MYL | 3 |
Radixin | RDX | 1 |
Tublins | TUB | 13 |
(4) Heat shock proteins and chaperones | ||
Heat shock cognate 71 kDa protein | HSPA8 | 1 |
T-complex protein 1 | CCT | 8 |
(5) Metabolic enzymes | ||
Aldolase A | ALDOA | 1 |
Fatty acid synthase | FASN | 1 |
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 1 |
Phosphoglycerate kinase 1 | PGK1 | 1 |
Phosphoglycerate mutase 1 | PGAM1 | 1 |
Pyruvate kinase isozymes M1/M2 | PKM2 | 1 |
(6) Multi vesicular body (MVB) biogenesis | ||
Alix | PDCD6IP | 1 |
ESCRT I complex | ||
Tumor susceptibility gene 101 protein | TSG101 | 1 |
Vacuolar sorting protein 28 | VPS28 | 1 |
Vacuolar protein sorting-associatedprotein 37 | VPS37 | 3 |
ESCRT II complex | ||
Vacuolar protein-sorting-associated protein 25 | VPS25 | 1 |
Vacuolar protein-sorting-associated protein 36 | VPS36 | 1 |
Vacuolar-sorting protein SNF8 | SNF8 | 1 |
ESCRT III complex | ||
Charged MVB proteins | CHMP | 2 |
(7) Signaling proteins | ||
14-3-3 Proteins | YWHA | 6 |
GTPase HRas | HRAS | 1 |
Rho GDP-dissociation inhibitor 1 | ARHGDIA | 1 |
Rho-related GTP-binding protein RhoC precursor | RHOC | 1 |
Ras-related protein Rap-1b | RAP1B | 1 |
Ras-related protein R-Ras2 | RRAS2 | 1 |
Ras GTPase-activating-like protein | IQGAP1 | 1 |
Syntenin-1 | SDCBP | 1 |
Transforming protein RhoA | RHOA | 1 |
Guanine nucleotide-binding protein (G proteins) | ||
-G(I)/G(S)/G(T) subunit beta | GNB | 4 |
Protein category and description Gene symbol | ||
-G(I)/G(S)/G(O) subunit gamma | GNG | 1 |
-G(S) subunit alpha | GNAS | 1 |
-subunit alpha | GNA | 4 |
-G(I), alpha | GNAI | 1 |
(8) Tetraspanins | ||
CD9 antigen | CD9 | 1 |
CD63 antigen | CD63 | 1 |
CD81 antigen | CD81 | 1 |
CD82 antigen | CD82 | 1 |
(9) Transcription and protein synthesis | ||
Histones | HIST | 14 |
Ribosomal proteins | RPS | 34 |
Ubiquitin | RPS27A | 1 |
Elongation factor 1-a 1 | EEF1A1 | 1 |
(10) Trafficking and membrane fusion | ||
Annexins | ANXA | 1 |
ADP-ribosylation factor | ARF | 3 |
AP-2 complex subunit α-1 | AP2A1 | 1 |
AP-2 complex subunit β-1 | AP2B1 | 1 |
Clathrin heavy chain 1 | CLTC | 1 |
Rab GDP dissociation inhibitor b | GDI2 | 1 |
Ras-related protein Rab | RAB | 9 |
Synaptosomal-associated protein 23 | SNAP23 | 1 |