Proteomics of human brain endothelial cell extracellular microvesicles (HBEC-EMVs). (A) Workflow of the EMV isolation from HBEC media. (B) Venn diagram showing the overlap among the identified proteins by 3 proteomics methods used for analysis of EMVs. FASP: filtered-aided sample preparation, gel-LC: gel electrophoresis followed by nanoLC-MS/MS, DR: detergent removal. See Methods for more details.