Fig. 3

Vessels along the arteriovenous (AV) axis during the acute phase of ischemic stroke. A Maximum intensity projection of cerebral vessels in Claudin5-GFP reporter mice depicting endothelial cells (green) and TRITC70 signal (red). A, artery; Ae, arteriole; C, capillaries; Ve, venule; V, vein. Scale bar 100 µm. B Pre M2CAO vessel diameter along the AV axis. C Distribution of leaky vessels along the AV axis during the acute phase after occlusion. n = 200 leaky vessels from 4 animals. D Quantification of TRITC70 extravasation from leaky vessels during the acute phase after occlusion. n = 200 leaky vessels from 4 animals. E Maximum intensity projection of the capillary (C) bed 140 min post M2CAO. TRITC70 leakage around an ascending venule (Ve, lumen outlined) and capillaries (arrowheads) can be observed. Scale bar 100 µm. F Example images of leaky capillaries at 90 min and 120 min post M2CAO (arrow heads). Scale bars 25 µm (Position 1) and 50 µm (Position 2). G Images as in (A) during the acute phase after M2CAO. Sites of leakage are marked with arrowheads. TRITC70 leakage distance is demarcated with dashed lines (g’-g’’’’). t1, time point 1; scale bars (G) 50 µm; scale bars (g’-g’’’’) 25 µm. H Quantification of TRITC70 extravasation from leaky vessels at t1 and t1 + 40 min; increased (dark green) and decreased (light green) leakage from individual vessels indicated, n = 100 leaking vessels from 4 animals, **p < 0.005 (two-tailed, paired t-test). I Example image of vasoconstriction in the peri-infarct region 120 min after M2CAO. Arterioles (Ae) marked in red; capillaries (C) marked in purple; venules (Ve) marked in blue. Scale bars (I) 25 µm, (i’-i’’’’’’) 10 µm. J Relative vessel diameter changes after M2CAO. n (artery) = 7; n (arteriole) = 14; n (capillary) = 113; n (venule) = 128; n (vein) = 16; from 4 animals