Fig. 2

Vascular leakage and perfusion in the acute phase after ischemic stroke. A Maximum intensity projections of two-photon imaged z-stacks of FITC70 at two alternating positions (schematic in a’) before and at different time points after M2CAO. Landmark vessels for orientation are outlined with dashed lines. M2CA and direction of blood flow are indicated (arrows). The location of the clot is marked. Asterisks indicate extravasated, parenchymal FITC70. Scale bars 100 µm. B Flow chart of three-dimensional (3D) leakage analysis using Simple Neurite Tracer (SNT) in ImageJ/Fiji. C Average FITC70 leakage before and after M2CAO. n = 4 animals. Two positions were imaged per animal. Colored lines indicate individual animals. **p < 0.01 (two-tailed, paired t-test). D FITC70 extravasation at different time points post M2CAO. n = 4 mice. Colored symbols indicate individual animals. E Average perfused vessel density before and after M2CAO. n = 4 animals. Two positions were imaged per animal. Colored lines indicate individual animals. *p < 0.05 (two-tailed, paired t-test). F Perfused vessel density at different time points post M2CAO. n = 4 mice. Colored symbols indicate individual animals