Fig. 3

ScRNA-seq analysis of the fourth ventricle CP identifies various CP-resident cell types. A UMAP of scRNA analysis of CP, colored by cluster identity. B UMAP of genes marking mesenchymal cells (Hic1), pericytes (Rgs5), fibroblasts (Mfap4), endothelial cells (Podxl), smooth muscle cells (Myocd), immune cells (Tnf), CP epithelium (Htr2c) and glia (Aqp4). The dotted lines indicate which Hic1⁺ cells were included in the aggregate for reanalysis in Fig. 5. C Heat-map of enriched transcripts within the identified CP clusters