Fig. 4

Analysis of human iPSC-BMELC barrier disruption based on RNA-seq. A Comprehensive RNA-seq was performed using total RNA with or without SARS-CoV-2 infection. Heatmap shows screened up- and downregulated genes by using cut-off values of fold change of ≥ 1.5 followed by second selection based on KEGG pathway analysis and references. B After SARS-CoV-2 infection for 24 h, tight junction marker genes (CLDN3, CLDN5, CLDN11, and CLDN12) were analyzed by RT-qPCR. C After SARS-CoV-2 infection for 24 h, expression of CLDN3 and CLDN11 was analyzed by western blot. Cropped blots were shown and the full-length blots were indicated in Fig. S5. The relative band densities were analyzed using ImageJ software and normalized to β-actin. Data are represented as mean ± SD (n = 3). *P < 0.05