Fig. 3

BMEC monolayer barrier examined after SARS-CoV-2 infection. A Cells were seeded in Transwell chambers. After the cells were infected with SARS-CoV-2 for 24 h, the TEER values across monolayers were measured. B The TEER values were measured at 0, 6, 12, 18 and 24 h after SARS-CoV-2 infection. C After SARS-CoV-2 infection for 24 h, the cells were stained with antibodies against cleaved caspase-3 (green) and SARS spike glycoprotein (red). Nuclei were counterstained with DAPI (blue). Bar = 20 μm. Data are represented as mean ± SD (n = 3). *P < 0.05