Fig. 1

SARS-CoV-2 infection examined in human iPSC-BMELCs. A Cells were infected with SARS-CoV-2 (MOI = 1) for 24 h. The intracellular viral copy number was determined by RT-qPCR. B After the cells were infected with SARS-CoV-2, viral titers in the culture supernatants were determined by plaque assay in TMPRSS2 expressed Vero E6 cells. C After 24 h of SARS-CoV-2 infection (MOI = 1), the cells were stained with antibodies against SARS-CoV-2 nucleocapsid protein (green) and SARS spike glycoprotein (red). Nuclei were counterstained with DAPI (blue). D After SARS-CoV-2 infection, the cells were stained with antibodies against CD31 (upper; green), CLDN5 (lower; green) and SARS CoV-2 spike glycoprotein (red). Nuclei were counterstained with DAPI (blue). Bar = 20 μm. Data are represented as mean ± SD (n = 3)