Fig. 7
Fasting increases MCT1 expression and activity in vivo. Rats were fasted for two days (F2) or fed ad libitum (AL) and dosed concomitantly with GSK3787 (GSK) (2 mg/kg i.p. every 12 h) or vehicle. (a) qRT-PCR analysis of Angptl4 relative gene expression in soleus muscle. Mean ± SD. Brown-Forsythe and Welch ANOVA followed by Dunnett’s multiple comparison tests. (b) Western-blot analysis of the relative protein expression of MCT1 in brain microvessels. For quantitative comparison, signals were normalized upon the signal from the endothelial-specific marker CD31. Mean ± SD. One-way ANOVA with Holm-Sidak’s multiple comparisons test. (c) Uptake clearance of [14C]-Lactate measured by brain in situ perfusion. Mean ± SD. One-way ANOVA with Holm-Sidak’s multiple comparisons test