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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Fasting upregulates the monocarboxylate transporter MCT1 at the rat blood-brain barrier through PPAR δ activation

Fig. 3

Effect of fasting on the expression of ABC transporters in brain microvessels and liver. Rats were fed ad libitum (AL) or fasted for one day (F1) or three days (F3). (a) Western-blot analysis of the expression of PGP, BCRP, and MRP4 in brain microvessels. For quantitative comparison, signals were normalized upon the signal from the endothelial-specific marker CD31. (b) Uptake clearance (Kin) of the PGP specific substrate [3H]-Digoxin as measured by in situ brain perfusion in AL rats, F1 rats and AL rats in the presence of the PGP-specific inhibitor Elacridar as a positive control. (c) Western-blot analysis of the expression of PGP in the liver. For quantitative comparison, the signal was normalized upon the signal from Actin. (d) qRT-PCR analysis of the relative gene expression of Abcb1a. (a-d) Mean ± SD. One-way ANOVA with Holm-Sidak’s multiple comparisons test

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Fluids and Barriers of the CNS

ISSN: 2045-8118