Fig. 4
From: Transient but not chronic hyperglycemia accelerates ocular glymphatic transport
In vivo magnetic resonance imaging revealing reduced CSF space in diabetic optic nerves. (A) Representative curved planar reconstruction of the optic nerve of representative control (4 months) and diabetic mice at two- or four-months after sham or STZ injection. Left: Longitudinal view, right: Cross section view. Green line indicate location of represented cross section view. Yellow arrow indicates diminished CSF filled subarachnoid space (SAS) in diabetic optic nerves. Scale bar: 200 μm. (B) Segmentation of the optic nerve into four segments of equal length: Segment 1 posterior pre-chiasmatic optic nerve part, segments 2 and 3: middle parts of the optic nerve, segment 4: anterior optic nerve part behind the orbit. C + D) Diagrams show the occupation rate (%) of the optic nerves (C) and the CSF-filled SAS (D) for each segment. n = 5, ***P ≤ 0.001, **P ≤ 0.01 between indicated groups by two-way ANOVA with Tukey’s correction. All graphs show mean ± SD