Fig. 6

Endothelial transcellular permeability is increased in α5-TPKO mice. A Representative images of MFSD2a (green) and Podocalyxin (red) staining in the cortex of 4 ∼ 6-month-old control and α5-TPKO mice (both sexes). Scale bar: 50 μm. B-D Quantifications of MFSD2a fluorescent intensity in the cortex (B), hippocampus (C), and striatum (D) of control and α5-TPKO mice. **p = 0.0079, Mann-Whitney U test. n = 5 mice. Data were shown as mean ± SD. E Representative images of Caveolin-1 (green) and Podocalyxin (red) staining in the cortex of 4 ∼ 6-month-old control and α5-TPKO mice (both sexes). Scale bar: 50 μm. F-H Quantifications of Caveolin-1 fluorescent intensity in the cortex (F), hippocampus (G), and striatum (H) of control and α5-TPKO mice. *p = 0.0159 and **p = 0.0079, Mann-Whitney U test. n = 5 mice. Data were shown as mean ± SD. I Representative TEM images showing endothelial transcytosis in 4 ∼ 6-month-old control and α5-TPKO mice (both sexes). Arrows indicate caveolae vesicles. PC, pericyte; EC, endothelial cell; Scale bar: 500 nm. J Quantifications of endothelial caveolae vesicle number in control and α5-TPKO brains. ****p < 0.0001, Mann-Whitney U test. n = 20–22 capillaries from 3 mice