Fig. 5

Endothelial paracellular mechanism is unaltered in α5-TPKO mice. A Representative images of ZO-1 (green), Claudin-5 (red), and Podocalyxin (magenta) staining in the cortex of 4 ∼ 6-month-old control and α5-TPKO mice (both sexes). Scale bar: 50 μm. B-G Quantifications of ZO-1 coverage (B, D, and F) and contact (C, E, and G) in the cortex (B and C), hippocampus (D and E), and striatum (F and G) of control and α5-TPKO mice. Non-significant, Mann-Whitney U test. n = 5 mice. Data were shown as mean ± SD. H-M Quantifications of Claudin-5 coverage (H, J, and L) and contact (I, K, and M) in the cortex (H and I), hippocampus (J and K), and striatum (L and M) of control and α5-TPKO mice. Non-significant, Mann-Whitney U test. n = 5 mice. Data were shown as mean ± SD. N Representative TEM images showing endothelial tight junctions in 4 ∼ 6-month-old control and α5-TPKO mice (both sexes). Arrows indicate tight junctions. PC, pericyte; EC, endothelial cell; Scale bar: 500 nm