Fig. 9
MRI analysis of CSF spaces and resistance to CSF drainage in testosterone-treated Zucker rats. A Brain water content in lean and testosterone-treated obese (obese + TT) Zucker rats, n = 4–6. B Brain weight and C relative brain weight of lean and obese Zucker rats, n = 4–6. D Brain volume obtained from the MRI scans, n = 4 of each. E-F Representative T2 MRI images from a lean (E) and an obese + TT (F) Zucker rats displaying total CSF spaces illustrated in green (lateral ventricles), dark blue (3rd ventricle), yellow (4th ventricle) or light blue (cisterna magna). G Total CSF space, H Lateral ventricle volume, and I 3rd ventricle volume obtained from the MRI scans, n = 4 of each. J representative ICP traces of lean and obese + TT Zucker rats undergoing resistance to CSF drainage experiments by infusion of aCSF at the rates indicated in the figure. K Resistance to CSF drainage obtained in lean and obese + TT Zucker rats, n = 6 of each group. L Correlation analysis of the resistance to CSF drainage as a function of bodyweight, n = 12. Statistical significance evaluated with Student’s unpaired t-test or simple linear regression and results shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant