Fig. 4

RNA expression in Zucker obese rats. A illustrates a pie chart of the percentage differentially expressed genes. B A heatmap of the differentially expressed genes in the individual Zucker rats, coloured based on the Z-score of normalized fold changes (n = 6 of each). C Volcano plot of all transcribed genes detected in the choroid plexus of lean and obese Zucker rats with differentially expressed genes (p < 0.05) marked in red (upregulated) or blue (downregulated). D GO enrichment analysis of the protein class categories. E Bar chart of all differentially expressed transporters in lean vs. obese Zucker rats. Bar height is expression levels in transcript per million (TPM) with significance levels (* p < 0.05, ** p < 0.01, and *** p < 0.001). F Association protein network analysis of the highest order cluster of genes. The protein nodes are coloured based on log2FC (downregulated in blue) and the connecting lines coloured based on the string confidence score from 0.7 (grey) to 1.0 (black). Size of the nodes are based on expression levels in four different categories