Fig. 5

Perivascular brain influx and drainage of Texas Red-conjugated ovalbumin into dcLNs. A, A brain section from a control mouse showing deep parenchymal penetration (arrow) and cortically located 45 kDa Texas Red-conjugated ovalbumin (arrowhead). B, A brain section of a mouse after BPL displays significantly reduced presence of tracer, as quantified in E. C, D, Representative images demonstrating that BPL (D) improves tracer uptake into to dcLNs compared to saline-treated controls (C). A – D, Blue, DAPI; Red, 45 kDa Texas Red-conjugated ovalbumin. E, F, Fluorescence intensity quantitation from serial brain sections and dcLNs, *P < 0.05, unpaired t-test with Welch’s correction and unpaired t-test, respectively. Scale bars for brain sections and dcLNs are 1 mm and 100 µm, respectively. A–F, n = 6 mice per group. Filled circles in E and F represent individual mice