Fig. 2

Manipulation of CSF tracer efflux with BPL. A, Experimental scheme and timeline to investigate the effect of BPL on CSF tracer efflux. 40 kDa FITC-dextran (FD40) injection into the cisterna magna (CM) CM was followed by blood sampling from femoral vein 5 min later, with an i.v. (femoral vein) infusion of nicardipine (Ncrd) or saline (Cntrl) starting 2 min after the first blood sampling for either 15 or 40 min, after which the final blood sampling was performed. B, C, FD40 concentration change in blood during 15-min and 40-min BPL, Mann–Whitney U test and unpaired t-test with Welch’s correction, respectively. D, Comparison of selected timepoints in control and BPL groups. Two-way ANOVA with Šidák’s multiple comparisons. B–D, *P < 0.05, ns = non-significant. n = 5–6 mice per group. Filled and open circles in B-D represent individual mice