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Table 2 Protein copy number and concentration following 24-h treatment with cocaine (10 μM) or vehicle as determined by proteomics analysis

From: Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier

Protein

Vehicle

Cocaine

Copy Number

Concentration (nM)

Copy Number

Concentration (nM)

CEACAM1

59,626 ± 10,789

43.9 ± 7.5

106,220 ± 7,458**

86.6 ± 4.4**

COL6A1

206,854 ± 12,118

152.2 ± 3.7

131,021 ± 14,120**

106.9 ± 11.7**

CTSB

8,484,178 ± 416,822

6,252.0 ± 355.4

6,593,383 ± 408,852**

5,377.0 ± 230.0*

HPX

6,543,012 ± 138,928

4,821.0 ± 139.0

4,814,035 ± 116,364****

3,931.0 ± 184.8**

ICAM

676,996 ± 14,380

499.0 ± 24.0

721,746 ± 23,606*

588.8 ± 7.0**

MIF

3,493,966 ± 119,612

2,573.0 ± 39.4

4,902,856 ± 535,590*

3,996.0 ± 358.4**

PECAM

1,202,038 ± 59,972

885.6 ± 46.24

956,743 ± 10,063**

780.8 ± 9.6*

PICALM

1,014,023 ± 21,188

747.1 ± 20.5

788,182 ± 30,189***

643.2 ± 25.7**

NRP2

287,851 ± 21,441

212.0 ± 13.9

186,651 ± 2,301**

152.3 ± 3.6**

VTN

1,208,330 ± 68,554

890.8 ± 65.2

862,826 ± 6,116***

704.3 ± 20.8**

VWF

584,018 ± 20,536

430.1 ± 9.7

484,891 ± 17,129**

395.6 ± 11.0*

  1. Mean ± standard deviation are shown. Asterisks indicate statistical significance in cocaine treated cells relative to vehicle. Paired T-test. COL6A1, Collagen Type VI Alpha 1 Chain. CTSB Cathepsin B. HPX Hemopexin. MIF Macrophage Migration Inhibitory Factor. PICALM Phosphatidylinositol Binding Clathrin Assembly Protein. NRP2 Neuropilin 2. VTN Vitronectin. VWF Von Willebrand Factor. *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001