Fig. 7

Cocaine Modulates Drug Transporter Expression. Immunofluorescent microscopy was performed to evaluate (A) BCRP, B OAT1, E ENT1, F OAT3, I MRP1, J OATP1A2, M MRP4, N OATP2A1, Q MRP5, or R P-gp (green) following treatment with cocaine (10 μM, right) or vehicle (left) for 24 h. DAPI was used to visualize nucleus (blue). One paired representative image, out of 20 individual images, are shown. All scale bars = 50 μm. (C, D, G, H, K, L, O, P, S, T) Quantification of the fluorescent signal from immunofluorescent microscopy was performed for endothelial cells treated with cocaine (10 μM, burgundy) or vehicle (teal) for 24 h. Twenty independent experiments (represented by individual dots) were performed. Estimation plots are shown where the left y-axis denotes relative fluorescent intensity (RFU, pixels) and the right y-axis reflects the effect size (black bar), which is the difference between means of each condition. Data are represented as mean ± standard deviation. *p < 0.05. ***p < 0.001. ****p < 0.0001. Unpaired T-test was performed