Fig. 1

Differential ART Propensity to Cross the BBB. FTC (grey), TFV (blue), and DTG (red) (all at 10 μM) were added to the apical portion of the BBB model and allowed to extravasate into the basolateral portion for 24 h at 37 °C, 5% CO₂. After this period of time, the media in the basolateral compartment was collected and the concentration of each ART drug that passed was measured by liquid chromatography/mass spectrometry. Dashed line denotes quantitative limit of detection for DTG. Three independent experiments (represented by individual dots), that included seven technical replicates, were performed. Data are represented as mean ± standard deviation. *p < 0.05. One-way ANOVA was performed