Fig. 6
From: Large molecules from the cerebrospinal fluid enter the optic nerve but not the retina of mice
Perivascular and parenchymal uptake of intrathecal antibodies in the optic nerve following cisternal injection. (A) Confocal images of optic nerve that was fixed 30 min. after cisternal injection of 70 kDa rhodamine dextran and AQP4-IgG at 5 µl/min, and then subsequently stained with antibodies to AQP4 and with anti-human IgG to detect the injected AQP4-IgG. (B) High resolution confocal z-sections showing distribution of AQP4 and AQP4-IgG at the optic nerve surface. Arrowheads demonstrate uptake of AQP4-IgG in perivascular spaces deep within the optic nerve. The relative depth of each optical section is indicated. (C) Ratio of AQP4-IgG to AQP4 staining intensity plotted against distance across the optic nerve along the line shown in the right panel of A. (D) Ratio of AQP4-IgG to AQP4 in 5 independent experiments measured in peripheral and central areas of optic nerve sections. * p < 0.05 by t-test, n = 5. (E) Distribution of injected AQP4-IgG and dextran in a section through the optic nerve head and retina, showing accumulation of AQP4-IgG in the sclera and connective tissues (arrowhead)