Fig. 5
From: Large molecules from the cerebrospinal fluid enter the optic nerve but not the retina of mice
Cisternal injection conditions determine the amount of therapeutic antibody that accumulates in the optic nerve. (A) Staining of optic nerve sections with anti-human secondary antibodies reveals uptake of human IgG throughout the optic nerve following high volume cisternal injections of human IgG and 70 kDa dextran tracer. (B–D) Quantification of the fluorescence intensity of IgG at the optic nerve surface, perivascular spaces and parenchyma of the optic nerve following high or low volume cisternal injection of a fixed amount of human IgG