Fig. 4
From: Large molecules from the cerebrospinal fluid enter the optic nerve but not the retina of mice
AQP4 is not required for uptake of cisternally-injected dextrans by the optic nerve. (A) Left Low magnification image showing fluorescence of 70 kDa dextran in acutely dissected optic nerves following high volume injection into the cisterna magna. Center and right panels. High magnification images showing distribution of co-injected 2000 kDa, 70 kDa and 10 kDa dextrans within the boxed regions shown at left. (B–D) Quantification of 70 kDa dextran intensity in the optic nerve sub-arachnoid space’ perivascular space and parenchyma from individual animals of each genotype (no significant difference in mean intensity was observed between genotypes by t-test (n = 8 each). (E) Tracer uptake in AQP4 expressing and non-expressing regions of the optic nerve. Frozen sections of the optic nerve head were prepared following high-volume injection with 70 kDa fluorescent dextran, then stained with antibodies to AQP4 and CD31. Tracer was observed in perivascular spaces of both regions that do (asterisk) and do not (arrowhead) express AQP4