Fig. 3
From: Large molecules from the cerebrospinal fluid enter the optic nerve but not the retina of mice
Fluid flow from the retina to the optic nerve via the perivascular spaces of the central retinal vessels (A) Experimental set up for determining the effect of elevated IOP on accumulation of cisternally injected dextrans in the optic nerve. (B) Measurements of intraocular pressure in eyes where pressure was acutely raised by anterior chamber cannulation (arrow) before and during cisternal injection of dextrans. Pressure measurements from 7 separate experiments are shown. (C) 70 kDa dextran tracer accumulation in the optic nerve head in a control eye or in the contralateral eye where IOP was elevated. (D) Relative fluorescent intensity of dextran tracer in control eyes and eyes where IOP was elevated (*p < 0.05 by paired t-test, n = 7). (E) Distribution of 3 different dextrans in the optic nerve head after intravitreal injection. The indicated dextrans were co-injected intravitreally and fixed by perfusion fixation 6 h later. Distribution of the dextrans in a slice through the optic nerve head is shown, the arrowhead indicates dextran accumulation around the central retinal vessels