Fig. 2
From: Large molecules from the cerebrospinal fluid enter the optic nerve but not the retina of mice
Cisternally injected solutes accumulate in the optic nerve head and sclera but not in the retina (A) Transverse section cut through the eye of a mouse after high volume cisternal injection of 70 kDa fixable TRITC-dextran following the protocol used in Fig. 1. Sections were subsequently stained with antibodies to CD31 (vascular endothelium). (B) Serial sections at 30 μm intervals through the optic nerve head from an animal injected and stained as in A. The arrowhead indicates cisternally injected dextran in the perivascular space surrounding the central retinal vessels in the optic nerve head but not further into the retina. (C) Distribution of 70 kDa tracer in a control eye (top) and in the contralateral eye (bottom) where intraocular pressure had been reduced by draining the anterior chamber. (D) Measurement of the minimum distance of visible dextran fluorescence from the retinal surface in control eyes and eyes with reduced IOP. No significant difference was observed