Fig. 6
Inflammatory phenotype of hCMEC/D3 in response to the size-based EV subpopulations. (A) Heat map and Z-score hierarchical clustering of inflammation-related protein expression (rows) in hCMEC/D3 treated with (~ 109) the size-based tEV subpopulations (2 K,10 and 100 K), TNFα/IFNγ treated cells and untreated cells. Clustering in rows and columns was done based on the Euclidean distance metric of 2–3 K-mean Value and a complete linkage method. The colors of the heat map indicate the highly expressed (red) and the basal expression levels (green) based on their Z-score. Euclidean distance also represents the close neighbors to the selected column (TNF-α– treated cells) according to their overall similarity. (B) Venn diagrams display the overlap of the expressed inflammatory proteins in hCMEC/D3 treated with (~ 109) of size- based tEV subpopulations (2 K,10 and 100 K) and TNFα/IFNγ treated cells. (C) Differential expression of inflammation-related proteins (log2 (fold change vs. ctrl)) in cells following tEV (~ 109) and inflammation stimulation