Fig. 4

Administration of size-based EV populations affect the EAE disease severity depending on their size. (A) Daily clinical scores and (B) weights were measures (n = 12 per group). Mice were i.v. treated with EV at onset of disease (vertical dotted line). (C) Sum of scores were analyzed, leaving out mice with a disease score of 0. (D) mRNA expression result on spinal cord collected on day 28 d.p.i. (E) Quantification of IgG/ laminin staining in the spinal cord of sEV and lEV injected mice compared to PBS injected mice, set as 100% (n = 3–4 / group). (F) Representative images showing IgG (green), laminin (red) and DAPI (blue). Statistical analysis was performed using a one-way ANOVA and Tukey’s multiple comparisons test with *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 in figures A-D. For figure E statistical analysis was performed using a two-tailed t-test with *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 after removal of two outliers using ROUT (Q = 1). Data are depicted as mean ± SEM