Fig. 2

Inflammatory proteome of the size-based EV populations in comparison with their corresponding parental cells (TNFα/IFNγ treated hCMEC/D3). (A) Heat map, K-means and Z-score hierarchical clustering of the relative abundance of each inflammatory protein (rows) in size-based fractionated EV, untreated hCMEC/D3 and TNFα/IFNγ treated hCMEC/D3 (columns) using the Euclidean distance metric and a complete linkage method. The colors of the heat map indicate the highly abundant (red) and the basal expression levels (green). Euclidean distance represents the close neighbors to the selected column (TNFα/IFNγ treated hCMEC/D3 (columns)) according to their overall similarity. (B) Venn diagram demonstrating the common and distinct differentially expressed inflammatory effectors in the EV of different sizes and TNFα/IFNγ treated hCMEC/D3 (columns). The significance was plotted in gray-red color scale, with gray indicating no significance and red high significance. (C) Top five most significantly enriched pathways of differentially expressed inflammatory proteins in the size-based fractionated tEV and corresponding parental cells (TNFα/IFNγ treated hCMEC/D3). Go terms were ranked based on the fold enrichment of -Log 10 (false discovery rate (FDR)) corrected p values. (D) Log 2 fold-change vs. Log 10 values of differentially expressed inflammatory proteins in size-based fractionated tEV and corresponding parental cells (TNFα/IFNγ treated hCMEC/D3)