Fig. 5

Distribution of small CSF tracers within the intracranial meninges. A Schematic showing location of meningeal, cerebral ventricular, and choroid plexus (ChP) structures highlighted in B–L. B Representative histology showing small CSF tracer (brown) distribution through the decalcified skull, underlying meninges (dura, arachnoid, and pia maters), and parenchyma 10 min after 1.9-nm gold nanoparticle (AuNP) injection into the right lateral ventricle of P7 rodents. scalebar = 50 µm. C Representative histology showing 1.9-nm AuNP distribution within the meningeal invaginations into the longitudinal fissure. The falx cerebri is labeled with a black arrow. C scalebar = 100 µm. D Quantification of the mean intensity of 1.9-nm AuNP distribution within the dura mater over the surface of the brain compared to the falx cerebri. Data are mean ± SD, n = 3 per group; Unpaired, two-tailed t-test. E–G Representative histology showing 1.9-nm AuNP distribution within the intracranial meninges of the ambient cistern (E), interpeduncular cistern (F), and choroidal fissure (G). E scalebar = 250 µm, F scalebar = 250 µm, G scalebar = 250 µm. H, I Representative histology showing the path the tela choroidea takes through the choroidal fissure from the roof of the third ventricle to the ChP of the lateral ventricle (H) (black arrowheads), and the tela choroidea pia mater as it gives rise to the right lateral ventricle ChP (H, I) (red arrowhead, H; inset I). H scalebar = 250 µm; I scalebar = 100 µm, I inset scalebar = 50 µm. J Representative histology of 1.9-nm AuNP distribution in the leptomeningeal invaginations into the folia of the cerebellum. J scalebar = 25 µm, J inset scalebar = 10 µm. K–M, 1.9-nm AuNP distribution in the choroidal fissure (K), ambient cistern (L), cerebellar folia (M), 10 min post-1.9-nm AuNP injection into the right lateral ventricle of rats with intraventricular hemorrhage-posthemorrhagic hydrocephalus (IVH-PHH, induced 72 h before AuNP injection with intraventricular hemoglobin (HB)) and aCSF control rats. K–M scalebars = 50 μm, J scalebars = 250 μm. Quantifications are also shown as mean ± SD with unpaired two-tailed t tests, n = 3 animals per group. All data are representative of 3 rodents