Fig. 5

Detection of the morphologically altered Hindbrain Choroid Plexus within the Tmem107 mutant embryos. A The visualization of the Hindbrain Choroid Plexus within the E15.5 WT (top horizontal panel) and Tmem107 KO (bottom horizontal panel) by the in situ analysis of the Hindbrain Choroid Plexus epithelial marker—Ttr (left vertical panel), single µCT slice (middle vertical panel) as well as 3D model (right vertical panel). Individual scale bars can be found within the images. B–C No statistically significant changes in the Hindbrain Choroid Plexus volume (B) or area (C) were detected by the analyses of E15.5 WTs and Tmem107 KOs. Note for B, C, D, E, G, I, L, N—the number of biological replicates within the particular group is indicated in the graphs, (WT—n = 5, Tmem107 KO—n = 3). Error bars indicate the standard deviation, p-values—unpaired two-tailed Student´s t-test. Note for F, H, J, K—The data points visualized together with standard deviations as the error bars within the graphs represent the mean of measured parameters from WT—n = 5, Tmem107 KO—n = 3, p = two-way ANOVA with Turkey´s multiple comparison test D An increase in the 4th ventricle volume was detected in Tmem107 KO compared to WT individuals. E No significant shift in the Hindbrain Choroid Plexus proportion in the 4th ventricle volume was observed analyzing the WT and Tmem107 KOs. F No statistically significant changes in the volume of the rostral and caudal part of E15.5 WTs and Tmem107 KOs were observed. G, H No statistically significant change in total outgrowth angle (G) was detected during the analysis of E15.5 Hindbrain Choroid Plexus WTs vs. Tmem107 KOs. Nonetheless, the left–right breakage symmetry was observed within this morphological parameter (H), I, J No statistically significant change in total length was detected during the analysis of E15.5 Hindbrain Choroid Plexus WTs vs. Tmem107 KOs (I), Additionally, no the left–right breakage symmetry was observed within this morphological parameter (J). K Quantification of the differences in the ventricle length, total Hindbrain Choroid Plexus length as well as the length of the central Hindbrain Choroid Plexus between the E15.5 WTs and Tmem107 KOs determining the slight increase in the length of the lateral segments of the Hindbrain Choroid Plexus, which are protruding into the lateral recesses of the 4th ventricle in Tmem107 KOs, compared to the WT littermates. L Quantification of the whole landmark analysis displays no significant shape change between Hindbrain Choroid Plexus E15.5 WTs and Tmem107 KO. M The illustration of the selected landmarks for the specific Landmark analysis for shape analysis of Tmem107 KOs compared to corresponding WTs. Landmarks are placed within the frontally positioned 3D model of E15.5 WT Hindbrain Choroid Plexus. Scale bar: 0.2 mm. N Quantification of the protrusion distance of the selected landmark presenting significant shape change within the lateral segments of the Hindbrain Choroid Plexus comparing E15.5 WTs and Tmem107 KO. 3D three dimensional, µCT X-ray micro-computed tomography, E embryonic day, I knockout, WT wild-type