Fig. 4

Detection of the morphologically altered Hindbrain Choroid Plexus of Cdk13 mutant embryos. A The visualization of the Hindbrain Choroid Plexus within the E13.5 WT (top horizontal panel), Cdk13 HM (middle horizontal panel) as well as Cdk13 KO (bottom horizontal panel) by the in situ analysis of the Hindbrain Choroid Plexus epithelial marker—Ttr (left vertical panel), single µCT slice (middle vertical panel) as well as 3D model (right vertical panel). Individual scale bars can be found within the images. B Hindbrain Choroid Plexus volume reduction was detected within the E13.5 Cdk13 KO mouse embryos compared to the WT as well as Cdk13 HM. Note for B, C, D, E, G, I, L—Individual data points representing biological replicates (WT—n = 5, Cdk13 HM—n = 3, Cdk13 KO—n = 3) and standard deviation are presented; statistical differences were analyzed by one-way ANOVA with Turkey´s multiple comparison test, and individual p values are indicated. Note for F, H, J, K—The data points visualized together with standard deviations as the error bars within the graphs represent the mean of measured parameters from WT—n = 5, Cdk13 HM—n = 3, Cdk13 KO—n = 3, p-values = two-way ANOVA with Turkey´s multiple comparison test C Quantification of the Hindbrain Choroid Plexus area differences between the E13.5 Hindbrain Choroid Plexus of WT, Cdk13 HM and Cdk13 KO, displaying the trend of decrease in this morphological parameter within the Cdk13 HM and primarily Cdk13 KO embryos. D An increase was detected in the 4th ventricle volume of Cdk13 KO compared to WT and Cdk13 HM individuals. E A strong reduction of the Hindbrain Choroid Plexus volume proportion in the 4th ventricle volume was observed within the Cdk13 KOs compared to the WT as well as Cdk13 HM embryos. F The disbalance in the Hindbrain Choroid Plexus outgrowth along the rostrocaudal axis was detected within the Cdk13 KO embryos comparing to the WT and Cdk13 HM G–H Quantification of the Hindbrain Choroid Plexus outgrowth angle differences between the E13.5 Hindbrain Choroid Plexus of WT, Cdk13 HM and Cdk13 KO, displaying the trend of increase in Cdk13 HM as well as Cdk13 KO embryos (G). However, no differences between the right and left Hindbrain Choroid Plexus branch outgrowth angles were detected (H). I–J The decrease in the Hindbrain Choroid Plexus length was observed within the Cdk13 KO groups compared to the WTs as well as Cdk13 HMs (I), while no disruption in the left–right symmetry was uncovered in this morphological parameter (J). K Quantification of the ventricle length; total Hindbrain Choroid Plexus length as well as the length of the central Hindbrain Choroid Plexus differences between the E13.5 WTs, Cdk13 HMs and Cdk13 KOs presenting the absence of the lateral parts of Hindbrain Choroid Plexus within the lateral recesses of the 4th ventricle of Cdk13 KOs, while the other measured parameters remain unchanged. L Quantification of the landmark analysis determined the large shape range between all analyzed samples (E13.5 Cdk13 HM and Cdk13 KO) compared to the corresponding WTs. More specifically, the low procrusion distance of landmarks placed within the Cdk13 HM was detected, whereas this parameter is significantly higher within the Cdk13 KO group. 3D three dimensional, µCT X-ray micro-computed tomography, E embryonic day, KO knockout, HM hypomorphic mutant, WT wild-type