Characterization of an iPSC-based barrier model for blood-brain barrier investigations using the SBAD0201 stem cell line

Table 2 Protein expression amounts of SLC and ABC transporters in crude membrane fractions of SBAD0201 cells (n = 8–9 biological replicates) prior to differentiation (D0) and after complete differentiation (D10) and in hCMEC/D3 cells (n = 7 biological replicates)

Transporter	SBAD0201 cells at D0 fmol/µg total protein mean ± SD	SBAD0201-derived BCECs at D10 fmol/µg total protein mean ± SD	hCMEC/D3 cells fmol/µg total protein mean ± SD
ABCB1/P-gp	< ULQ	< ULQ	0.24 ± 0.05
ABCG2/BCRP	< ULQ	0.18 ± 0.094	0.27 ± 0.13
ABCC1/MRP1	0.18 ± 0.066	0.11 ± 0.048	0.21 ± 0.07
ABCC4/MRP4	0.038 ± 0.016	0.021 ± 0.0097	0.026 ± 0.0088
SLC2A1/GLUT1	9.7 ± 2.2	6.9 ± 2.7	3.0 ± 1.0
SLC7A1/CAT1	1.2 ± 0.29	0.27 ± 0.069	0.36 ± 0.11
SLC7A5/LAT1	0.29 ± 0.14	0.11 ± 0.034	0.14 ± 0.07
SLC3A2/4F2hc	1.3 ± 0.65	0.53 ± 0.21	0.18 ± 0.085
SLC16A1/MCT1	0.23 ± 0.19	0.11 ± 0.073	0.040 ± 0.024
SLC27A1/FATP1	< ULQ	< ULQ	0.048 ± 0.024

ULQ – under limit of quantification. The protein expression was considered below the limit of quantification (LOQ) if signal peaks were obtained for only two or one MRM transition(s). The LOQ was estimated as the lowest concentration of a stable isotope-labelled peptide (spiked into the sample containing 50 µg of total protein followed by processing similarly as the study samples) for which signal peaks were achieved by three or more transitions

ISSN: 2045-8118