Table 1 Overview of antibodies used in the present study
Target protein | Clonality | Host | Dilution factor | Supplier and catalogue ID |
|---|---|---|---|---|
Primary antibodies | ||||
VE-Cadherin | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
PECAM-1 | Polyclonal | Mouse | 1:100 | Lundbeck A/S |
Von Willebrand Factor | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
Occludin | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
Claudin-5 | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
Zonula occludens-1 | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
Glut-1 | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
LAT-1 | Polyclonal | Rabbit | 1:100 | Bioss Antibodies (bs-10125R) |
P-glycoprotein | Polyclonal | Rabbit | 1:100 | Lundbeck A/S |
LRP-1 | Monoclonal | Rabbit | 1:100 | Abcam (Ab92544) |
Secondary antibodies conjugated with Alexa-488 or HRP | ||||
Anti-rabbit | Polyclonal | Goat | 1:200 | Life Technologies (AV1008) |
Anti-mouse | Polyclonal | Goat | 1:200 | Invitrogen (AH017) |