Fig. 2

Functional barrier integrity of SBAD0201-derived BCECs. (A) TEER across SBAD0201-derived BCEC monolayers was investigated in EC-/- medium and upon change to transport buffer (HBSS) with and without glucose. TEER was measured on an experimental day after 20 min equilibration at room temperature on shaking table and after changing to the transport buffer (with and without glucose, 10 min equilibrium at shaking table). Data are shown as mean ± SEM of three individual passages of six replicates. Statistics: One-way ANOVA with posthoc Tukey test, ****: p < 0.00005. (B) Transport rate of paracellular and transcellular transport markers in SBAD0201-derived BCECs. Transport studies were performed over two hours for the small markers, propranolol, mannitol, and sodium fluorescein (NaF). The transport of FITC-dextran (4 kDa) was followed for four hours. Data are shown as mean ± SEM of three independent flux experiments of three technical replicates (n = 3, N = 3)