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Table 1 Primary antibody information

From: Structural characterization of SLYM—a 4th meningeal membrane

Antigen

Immunogen

Manufacturer (CAT#)

Host & isotype

Dilution

Reference

RRID#

 

Aquaporin-1

(AQP-1)

19-aa peptide of Rat AQP1

Target epitope is in C-terminus, intracellular

Alpha Diagnostics (AQP11-A)

Rabbit IgG

1:800

AB_1609286

 

Aquaporin-4

(AQP-4)

Synthetic peptide of human Aquaporin 4 (C-terminal)

Aviva Systems Technology (OABB01958)

Rabbit IgG

1:3000

  

Cellular retinoic acid-binding protein 2 (CRABP2)

CRABP2 fusion protein Ag0309

Proteintech (10225-1-AP)

Rabbit IgG

1:200

AB_2085455

 

Claudin-11

(CLDN-11)

Synthetic peptide of human oligodendrocyte specific protein (C terminal)

Abcam (ab53041)

Rabbit IgG

1:400

AB_2276205

 

Dipeptidyl peptidase IV

(DPP4)

Mouse myeloma cell line NS0-derived

recombinant mouse DPPIV/CD26

Ser29-His760

Accession # P28843

R&D Systems (AF594)

Goat IgG

1:200

AB_355739

 

E-Cadherin

(E-Cad)

Recombinant full length protein

Abcam (ab231303)

Mouse IgG1

1:100

AB_2923285

 

Fibroblast Antibody

(ER-TR7)

Isolated C3H thymic stromal cells

Novus (NB100-64932)

Rat IgG2a

1:300

AB_963381

 

Green fluorescent protein

(GFP)

GFP isolated directly from Aequorea victoria

Thermo Fischer Scientific

(A-6455)

Rabbit IgG

1:1000-1:2000

AB_221570

 

Lymphatic vessel endothelial hyaluronan receptor 1

(LYVE 1)

Recombinant fragment His-Tag corresponding to Mouse LYVE1 aa 1-250 (C terminal)

Abcam (ab14917)

Rabbit IgG

1:100

AB_301509

 

Plectin

[E398P]

Recombinant fragment

Abcam (ab32528)

Rabbit IgG

1:250

AB_777339

 

Podoplanin

(PDPN)

Murine thymic stromal cell lines

Thermo Fischer Scientific (14-5381-85)

Syrian Hamster IgG

1:200

AB_1210507

 

Prospero homeobox 1 (Prox1)

Recombinant fragment

Abcam (ab199359)

Rabbit IgG

1:200

AB_2868427

 

Retinaldehyde dehydrogenase 2

(RALDH2)

Retinal dehydrogenase 2 recombinant protein epitope signature tag (PrEST)

Sigma Life Science (HPA010022)

Rabbit IgG

1:500

AB_1844723