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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Structural characterization of SLYM—a 4th meningeal membrane

Fig. 6

SLYM encases brain parenchyma evident in a coronal section through mid-pontine region. Immunohistochemical analysis of GFP signal of a coronal section of Prox1-eGFP tissue allowed additional characterization of the meningeal layers covering the parenchyma. SLYM (GFP+) layer divides the subarachnoid space into two well-defined compartments, an outer subarachnoid space (oSAS) facing the arachnoid barrier cell layer (ABCL) and an inner subarachnoid space (iSAS), enclosing blood vessels, here the basilar artery (BA). SLYM seems to fuse with pia covering a fraction of the basolateral part of pons on both sides (arrows) thus creating a sub-compartmentalization of the inner SAS. The strongly stained cisterna ambiens (CiA) is indicated by arrowheads. ABCL: arachnoid barrier cell layer, BA: basilar artery, CiA: cisterna ambiens, CiP: cisterna pontis, iSAS: inner subarachnoid space, oSAS: outer subarachnoid space. Magnification is indicated by labelled bar

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