Fig. 1
From: Structural characterization of SLYM—a 4th meningeal membrane
Preparation of histological sections is linked to a marked shrinkage of the brain’s fluid-filled spaces. (A) Magnetic resonance imaging (MRI) of a young isoflurane anesthetized C57BL/6JRj mouse. Bright white signal represents CSF in the subarachnoid space (SAS), perivascular spaces (PVS) and the ventricles which gradually disappears after death, indicative of brain swelling. After the live imaging session, the anesthetized mouse was killed by 100% nitrogen inhalation via a nose cone on an MR-compatible stereotactic holder, and scanning was repeated at 1, 6, 12, 18, and 24 h after cardiorespiratory arrest. (B) Histological section of the head of a young C57BL/6JRj mouse after decalcification and preparation of parafin. (C) Quantification of the volume of the brain, SAS/PVS, ventricles and total intracranial volume based on the images displayed in panel A. (D) Immunohistochemical analysis of arachnoid barrier cell layer (top, arrowheads) stained for Cld-11 and SLYM stained for GFP (lower, arrows) in two consecutive sections prepared from a Prox1-eGFP mouse. Note the considerable shrinkage of the brain (asterisk) in the sections