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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Application of a new MDCKII-MDR1 cell model to measure the extent of drug distribution in vitro at equilibrium for prediction of in vivo unbound brain-to-plasma drug distribution

Fig. 5

A depicts the relationship between log-transformed in vitro and in vivo derived Kp,uu,brain values for selected reference compounds (n = 12) using the Uni-L setup. B compares Kp,uu,brain values using predicted and in vitro determined free fractions in the experimental matrices. The in vitro data represents the mean ± SD of three passages conducted on three individual filters (n = 3, total N = 9), while the in vivo data represents three minipigs (n = 1, total N = 3). ‘n’ denotes test occasions, and ‘total N’ denotes the total number of replicates. The solid lines represent the line of unity and a twofold difference, and the dotted lines represent different levels of brain penetration: restricted (Kp,uu,brain < 0.3), partially restricted (Kp,uu,brain between 0.3 and 0.7), and unrestricted (Kp,uu,brain > 0.7)

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Fluids and Barriers of the CNS

ISSN: 2045-8118