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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Application of a new MDCKII-MDR1 cell model to measure the extent of drug distribution in vitro at equilibrium for prediction of in vivo unbound brain-to-plasma drug distribution

Fig. 3

The concentration–time profiles for three exemplified compounds using Uni-L (loading compound apically) in A–C and Bi-L (loading compound into both compartments) in D–F. Antipyrine is a passive permeable compound, cimetidine is a P-gp substrate, and diphenhydramine is a potential uptake substrate. Concentrations in the apical (black circles) and basolateral compartments (open circles) of the in vitro Kp,uu,brain experiment are shown, compounds were loaded at nominal concentrations of 1 μM. Data represents mean ± SD of three occasion/cell passages of three individual filters, ‘n’ denotes test occasions, and ‘total N’ denotes the total number of replicates (n=3, total N=9)

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Fluids and Barriers of the CNS

ISSN: 2045-8118