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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Application of a new MDCKII-MDR1 cell model to measure the extent of drug distribution in vitro at equilibrium for prediction of in vivo unbound brain-to-plasma drug distribution

Fig. 1

A The conventional permeability setup is shown, where discrete compounds were dosed in either the apical compartment or to the basolateral compartment, using transport buffer as matrice and a short incubation time of 1 h. B The in vitro equilibrium drug extent setup investigated in this publication, using BSA and brain homogenate and an incubation time of 29 h. Two different setups were investigated, either cassetted drugs were loaded to the apical chamber (into BSA, like a standard in vivo experiment) called the Uni-L method or they were added in both chambers called the Bi-L method

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Fluids and Barriers of the CNS

ISSN: 2045-8118