Fig. 2

Identification of phage-displayed peptides selectively retained on the surface of endothelial cells. Sequential bio-panning steps of an M13-phage-bound peptide library were carried out on primary endothelial cells derived from rat lung, liver or brain to identify peptides which either bind to each endothelial cell type (binding population), or which bind and are retained on the cell surface after an 8 h time-period (retained population). Peptide recovery after each selection round (bio-panning step) was measured by quantifying phage concentration (PFU) (a). Progressive selection of peptides retained on the cell surface was assessed by the ratio of recovered peptides between binding/retained populations after each selection round (b). The composition of the final peptide population was assessed by sequencing the DNA of individual phage clones after the third bio-panning step (c). Population similarities were assessed by comparing sequences shared between each endothelial phenotype (d). *Denotes the VSWP peptide found on brain shared 50% sequence homology with VSVP peptide found on lung and liver