Fig. 1
The extracellular diffusion of TMR is significantly increased in Tg2576 than wild-type mice. a, Maximum intensity projections (MIPs) of TMR-perfused vascular tree in the cortices of WT mice (top) and Tg2576 mice (bottom) mice 5 and 30 min after impalement. b, A single frame of a capillary in a wild-type cortical slice at 5 and 30 min (left) and the corresponding spatial fluorescence profiles (right) of a line profile normalized to the intravascular fluorescence. The analyzed line profiles (grey, 10-µm thick) were within 10 μm in a 3-µm distance to the sides. The extravascular fluorescence was 1.3 ± 0.03% at 30 min (the dashed bar region was averaged for quantification, see inset). c, A single frame of a capillary in Tg2576 cortex recorded at t5 and t30 (left) and the corresponding spatial fluorescence profiles (right) of a line profile. After 30 min, the extravascular fluorescence rose to 4.2 ± 0.04% (the dashed bar region was averaged for quantification, see inset). d, Quantification of extravascular fluorescence intensities within the specified ROIs across capillaries, 100–120 μm away from the impalement point, in WT and Tg2576 mice at 30 min. Data are represented as mean ± SEM. All intensities were background corrected and normalized to the intravascular fluorescence. The asterisk denotes a statistically significant difference (Student’s t-test, *P < 0.05). Data were obtained from 10 injections/4 animals (nROI WT mice = 8, nROI Tg2576 mice = 8)