Fig. 1

Sspo^−/− mice show a normal SCO but lack RF positive material in the SCO and ventricles. A Schematic depicting gene editing strategy. B Schematic depicting SCO location in sagittal brain sections corresponding to C. C BaseScope reveals lack of Sspo transcripts in the SCO of Sspo^−/− mice (12 weeks). Scale = 1 mm (left panels) and 200 µm (right panels). D Schematic depicting the brain sectioning strategy taken to visualize SCO ependyma and proximal ventricular region. E RF positive material was not detected in Sspo^−/− mice vs. controls. Scale = 50 µm