Fig. 1

Effect of PQQ treatment on folate transporters RFC (Slc19a1) and PCFT (Slc46a1) gene expression in primary cultures of mouse mixed glial cells. A RFC gene expression was significantly increased in both control and FD cells following 24- and 48- hours PQQ treatment (5 µm). B PCFT gene expression was significantly lower in FD cells compared to control cells. PCFT gene expression was significantly increased in both control and FD cells following 24- and 48-h PQQ treatment (5 µm). Results are presented as mean relative mRNA expression compared to the DMSO vehicle control ± SEM normalized to the housekeeping gene cyclophilin B from N = 5 independent primary cultures of mouse mixed glial preparations. One-way ANOVA with Bonferroni’s post-hoc test. Asterisks represent significant differences (*P < 0.05, **P < 0.01, ***P < 0.001)