Fig. 6

The effect of ammonia and TNFα on occludin and integrin β1 in a rat brain endothelial cell line (RBE4). A Protein level of occludin and integrin β1 with representative immunoblots. B Confocal microscopy analysis of occludin immunofluorescence (left panel, red), integrin β1 (right panel, red) in RBE4 cells treated or not with ammonia and TNFα. DAPI staining (blue). The results are the mean ± SD; n = 5; *p < 0.05 vs. control, **p < 0.01 vs. control, ***p < 0.01 vs. control, ****p < 0.01 vs. control, one-way ANOVA, and Dunnett’s post hoc test. The results are the mean ± SD. n = 5; *p < 0.05 vs. control, one-way ANOVA, Dunnett’s post hoc test